A Universal Protocol for Photochemical Covalent Immobilization of Intact Carbohydrates for the Preparation of Carbohydrate Microarrays

被引：43

作者：

Wang, Huibin ^{[1
]}

Zhang, Yiming ^{[1
]}

Yuan, Xun ^{[1
]}

Chen, Yi ^{[1
,2
]}

Yan, Mingdi ^{[3
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China

[2] Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

[3] Portland State Univ, Dept Chem, Portland, OR 97207 USA

来源：

BIOCONJUGATE CHEMISTRY | 2011年 / 22卷 / 01期

关键词：

OLIGOSACCHARIDE MICROARRAYS; PERFLUOROPHENYL AZIDES; GLYCAN ARRAY; SURFACE; BINDING; RECOGNITION; FABRICATION; REAGENTS; CHIPS; FILMS;

D O I：

10.1021/bc100251f

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A universal photochemical method has been established for the immobilization of intact carbohydrates and their analogues, and for the fabrication of carbohydrate microarrays. The method features the use of perfluorophenyl azide (PFPA)-modified substrates and the photochemical reaction of surface azido groups with printed carbohydrates. Various aldoses, ketoses, nonreducing sugars such as alditols, and their derivatives can be directly arrayed on the PFPA-modified chips. The lectin-recognition ability of arrayed mannose, glucose, and their oligo- and polysaccharides were confirmed using surface-plasmon resonance imaging and laser-induced fluorescence imaging.

引用

页码：26 / 32

页数：7