Protein changes during programmed cell death in tobacco

被引：6

作者：

Chaves, I. ^{[1
]}

Alves, M. ^{[1
]}

Carrilho, D. ^{[1
]}

Duque-Magalhaes, M. C. ^{[1
,2
]}

Ricardo, C. P. ^{[1
,2
]}

Regalado, A. P. ^{[1
]}

机构：

[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal

[2] Univ Tecn Lisboa, Inst Super Agron, P-1349017 Lisbon, Portugal

来源：

BIOLOGIA PLANTARUM | 2011年 / 55卷 / 01期

关键词：

heat shock proteins; calreticulin; Nicotiana tabacum BY-2 suspension cells; Yariv reagent; TRACHEARY ELEMENT DIFFERENTIATION; HYPERSENSITIVE RESPONSE; HYDROGEN-PEROXIDE; GENE-EXPRESSION; YARIV REAGENT; APOPTOSIS; HSP70; MITOCHONDRIA; CALCIUM; SENSITIVITY;

D O I：

10.1007/s10535-011-0021-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Programmed cell death (PCD) was induced by the Yariv reagent in Nicotiana tabacum cv. Bright Yellow-2 cell suspension. The analyses of proteins extracts by 2-D electrophoresis clearly show massive protein degradation which was mainly due to cysteine protease activity. In contrast, some proteins remained unchanged up to 72 h after PCD induction. Peptide mass fingerprints of these proteins, obtained by MALDI-TOF, identified calreticulin, heat shock protein (HSP) 60, HSP70, malate dehydrogenase and mitochondrial ATP synthase beta-subunit.

引用

页码：153 / 158

页数：6

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