Isolation and Characterization of Antifungal Peptides Produced by Bacillus amyloliquefaciens LBM5006

被引：62
作者：
Benitez, Lisianne Brittes [1 ]
Velho, Renata Voltolini [1 ]
Lisboa, Marcia Pagno [1 ]
da Costa Medina, Luis Fernando [1 ]
Brandelli, Adriano [1 ]
机构：
[1] Univ Fed Rio Grande do Sul, ICTA, Dept Ciencia Alimentos, Lab Bioquim & Microbiol Aplicada, BR-91501970 Porto Alegre, RS, Brazil
来源：
JOURNAL OF MICROBIOLOGY | 2010年 / 48卷 / 06期
关键词：
B; amyloliquefaciens; bacteriocin; antifungal activity; lipopeptide; DESORPTION/IONIZATION MASS-SPECTROMETRY; ITURIN-A; SUBTILIS; BACTERIOCIN; ANTIBIOTICS; SURFACTIN; LIPOPEPTIDES; SUBSTANCE; IDENTIFICATION; BIOCONTROL;
D O I：
10.1007/s12275-010-0164-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Bacillus amyloliquefaciens LBM 5006 produces antagonistic activity against pathogenic bacteria and phytopathogenic fungi, including Aspergillus spp., Fusarium spp., and Bipolaris sorokiniana. PCR analysis revealed the presence of ituD, but not sfp genes, coding for iturin and surfactin, respectively. The antimicrobial substance produced by this strain was isolated by ammonium sulfate precipitation, gel filtration chromatography and 1-butanol extraction. The ultraviolet spectrum was typical of a polypeptide and the infrared spectrum indicates the presence of peptide bonds and acyl group(s). The antimicrobial substance was resistant to proteolytic enzymes and heat treatment, and was reactive with ninhydrin. Mass spectroscopy analysis indicated that B. amyloliquefaciens LBM 5006 produces two antimicrobial peptides, with main peaks at ink 1,058 Da and 1,464 Da, corresponding to iturin-like and fengycin-like peptides, respectively. B. amyloliquefaciens LBM 5006 showed significant activity against phytopatogenic fungi, showing potential for use as a biocontrol agent or production of antifungal preparations.
引用
收藏
页码：791 / 797
页数：7
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