Induction of bone morphogenetic protein-2 by interleukin-1 in human fibroblasts

被引：20

作者：

Fowler, MJ ^{[1
]}

Neff, MS ^{[1
]}

Borghaei, RC ^{[1
]}

Pease, EA ^{[1
]}

Mochan, E ^{[1
]}

Thornton, RD ^{[1
]}

机构：

[1] Philadelphia Coll Osteopath Med, Dept Biochem & Mol Biol, Philadelphia, PA 19131 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 248卷 / 03期

关键词：

D O I：

10.1006/bbrc.1998.8988

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rheumatoid arthritis and periodontitis are chronic inflammatory diseases associated with tissue destruction that is mediated in part by elevated levels of cytokines (e.g., interleukin-1 and tumor necrosis factor). Differential screening of a human synovial fibroblast cDNA library for interleukin-1 induced genes revealed a clone identical to the gene encoding human bone morphogenetic protein-a. Northern blot analysis of human synovial fibroblast mRNA confirmed up-regulation of bone morphogenetic protein-2 in the presence of interleukin-1. Utilizing a specific antibody, levels of bone morphogenetic protein-2 protein in conditioned medium from synovial fibroblasts were also up-regulated in the presence of interleukin-1. This is the first report of the production of bone morphogenetic protein-a by synovial fibroblasts, and the first report of its up-regulation in response to interleukin-1. However, interleukin-1 did not induce bone morphogenetic protein-a mRNA in human gingival fibroblasts. (C) 1998 Academic Press.

引用

页码：450 / 453

页数：4

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