QuasR: quantification and annotation of short reads in R

被引:216
作者
Gaidatzis, Dimos [1 ,2 ]
Lerch, Anita [1 ,2 ]
Hahne, Florian [3 ]
Stadler, Michael B. [1 ,2 ]
机构
[1] Friedrich Miescher Inst Biomed Res, CH-4058 Basel, Switzerland
[2] Swiss Inst Bioinformat, CH-4058 Basel, Switzerland
[3] Novartis Inst Biomed Res, CH-4057 Basel, Switzerland
关键词
DIFFERENTIAL EXPRESSION ANALYSIS; BIOCONDUCTOR PACKAGE;
D O I
10.1093/bioinformatics/btu781
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A Summary: QuasR is a package for the integrated analysis of high-throughput sequencing data in R, covering all steps from read preprocessing, alignment and quality control to quantification. QuasR supports different experiment types (including RNA-seq, ChIP-seq and Bis-seq) and analysis variants (e.g. paired-end, stranded, spliced and allele-specific), and is integrated in Bioconductor so that its output can be directly processed for statistical analysis and visualization.
引用
收藏
页码:1130 / 1132
页数:3
相关论文
共 9 条
[1]   Differential expression analysis for sequence count data [J].
Anders, Simon ;
Huber, Wolfgang .
GENOME BIOLOGY, 2010, 11 (10)
[2]   Detection of splice junctions from paired-end RNA-seq data by SpliceMap [J].
Au, Kin Fai ;
Jiang, Hui ;
Lin, Lan ;
Xing, Yi ;
Wong, Wing Hung .
NUCLEIC ACIDS RESEARCH, 2010, 38 (14) :4570-4578
[3]   Bioconductor: open software development for computational biology and bioinformatics [J].
Gentleman, RC ;
Carey, VJ ;
Bates, DM ;
Bolstad, B ;
Dettling, M ;
Dudoit, S ;
Ellis, B ;
Gautier, L ;
Ge, YC ;
Gentry, J ;
Hornik, K ;
Hothorn, T ;
Huber, W ;
Iacus, S ;
Irizarry, R ;
Leisch, F ;
Li, C ;
Maechler, M ;
Rossini, AJ ;
Sawitzki, G ;
Smith, C ;
Smyth, G ;
Tierney, L ;
Yang, JYH ;
Zhang, JH .
GENOME BIOLOGY, 2004, 5 (10)
[4]   Ultrafast and memory-efficient alignment of short DNA sequences to the human genome [J].
Langmead, Ben ;
Trapnell, Cole ;
Pop, Mihai ;
Salzberg, Steven L. .
GENOME BIOLOGY, 2009, 10 (03)
[5]   Software for Computing and Annotating Genomic Ranges [J].
Lawrence, Michael ;
Huber, Wolfgang ;
Pages, Herve ;
Aboyoun, Patrick ;
Carlson, Marc ;
Gentleman, Robert ;
Morgan, Martin T. ;
Carey, Vincent J. .
PLOS COMPUTATIONAL BIOLOGY, 2013, 9 (08)
[6]  
Li H, 2009, BIOINFORMATICS, V25, P1094, DOI [10.1093/bioinformatics/btp324, 10.1093/bioinformatics/btp100]
[7]   The Subread aligner: fast, accurate and scalable read mapping by seed-and-vote [J].
Liao, Yang ;
Smyth, Gordon K. ;
Shi, Wei .
NUCLEIC ACIDS RESEARCH, 2013, 41 (10) :e108
[8]   ShortRead: a bioconductor package for input, quality assessment and exploration of high-throughput sequence data [J].
Morgan, Martin ;
Anders, Simon ;
Lawrence, Michael ;
Aboyoun, Patrick ;
Pages, Herve ;
Gentleman, Robert .
BIOINFORMATICS, 2009, 25 (19) :2607-2608
[9]   edgeR: a Bioconductor package for differential expression analysis of digital gene expression data [J].
Robinson, Mark D. ;
McCarthy, Davis J. ;
Smyth, Gordon K. .
BIOINFORMATICS, 2010, 26 (01) :139-140