Molecularly imprinted solid-phase extraction of (-)-ephedrine from Chinese Ephedra

被引：56

作者：

Dong, XC ^{[1
]}

Wei, WA

Ma, SJ

Sun, H

Li, Y

Guo, JQ

机构：

[1] Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China

[2] Shan Xi Normal Univ, Dept Chem, Linfen 041004, Peoples R China

[3] Tianjin Municipal Inst Drug Control, Tianjin 300070, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1070卷 / 1-2期

关键词：

molecularly imprinted solid phase extraction; (-)-ephedrine; Chinese Ephedra; sample clean-up;

D O I：

10.1016/j.chroma.2005.03.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Method of molecularly imprinted solid phase extraction (MISPE) of (-)-ephedrine from Chinese Ephedra has been developed in the research. The molecularly imprinted polymer (MIP) with good selectivity and affinity for (-)-ephedrine was synthesized with (-)-ephedrine as the template, methacrylic acid as the functional monomer. The washing and elution conditions in MISPE were selected and optimized for efficient analyte extraction and sample clean-up. A clean analytical HPLC base line of ephedra extract was obtained after MISPE, which indicated that the sample pre-treatment was efficient. Good recovery and precision were obtained in the assessment for the MISPE-HPLC procedure, which demonstrated it is a reliable method and can be used for the determination of (-)-ephedrine in herbal ephedra. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：125 / 130

页数：6

共 18 条

[1] Electrochemical sensors based on molecularly imprinted polymers [J].

Blanco-López, MC ;

Lobo-Castañón, MJ ;

Miranda-Ordieres, AJ ;

Tuñón-Blanco, P .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2004, 23 (01) :36-48

[2] Use of molecularly imprinted polymers in the solid-phase extraction of clenbuterol from animal feeds and biological matrices [J].

Brambilla, G ;

Fiori, M ;

Rizzo, B ;

Crescenzi, V ;

Masci, G .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2001, 759 (01) :27-32

[3] Optimization of the class-selective extraction of triazines from aqueous samples using a molecularly imprinted polymer by a comprehensive approach of the retention mechanism [J].

Chapuis, F ;

Pichon, V ;

Lanza, F ;

Sellergren, S ;

Hennion, MC .

JOURNAL OF CHROMATOGRAPHY A, 2003, 999 (1-2) :23-33

[4]

de Boer T, 2002, ELECTROPHORESIS, V23, P1296, DOI 10.1002/1522-2683(200205)23:9<1296::AID-ELPS1296>3.0.CO

[5]

2-2

[6] Separation of ephedrine stereoisomers by molecularly imprinted polymers -: influence of synthetic conditions and mobile phase compositions on the chromatographic performance [J].

Dong, XC ;

Sun, H ;

Lü, XY ;

Wang, HB ;

Liu, SX ;

Wang, N .

ANALYST, 2002, 127 (11) :1427-1432

[7] Comparison of various reversed-phase columns for the simultaneous determination of ephedrines in urine by high-performance liquid chromatography [J].

Imaz, C ;

Navajas, R ;

Carreras, D ;

Rodríguez, C ;

Rodríguez, AF .

JOURNAL OF CHROMATOGRAPHY A, 2000, 870 (1-2) :23-28

[8] Evaluation of a multidimensional solid-phase extraction platform for highly selective on-line cleanup and high-throughput LC-MS analysis of triazines in river water samples using molecularly imprinted polymers [J].

Koeber, R ;

Fleischer, C ;

Lanza, F ;

Boos, KS ;

Sellergren, B ;

Barceló, D .

ANALYTICAL CHEMISTRY, 2001, 73 (11) :2437-2444

[9] Molecularly imprinted solid phase extraction for rapid screening of cephalexin in human plasma and serum [J].

Lai, EPC ;

Wu, SG .

ANALYTICA CHIMICA ACTA, 2003, 481 (02) :165-174

[10]

LI H, 2001, CHIN J CHROMATOGR, V19, P161

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