The Conserved Splicing Factor SUA Controls Alternative Splicing of the Developmental Regulator ABI3 in Arabidopsis

被引：107
|
作者：
Sugliani, Matteo [1 ]
Brambilla, Vittoria [1 ]
Clerkx, Emile J. M. [2 ]
Koornneef, Maarten [1 ,2 ]
Soppe, Wim J. J. [1 ]
机构：
[1] Max Planck Inst Plant Breeding Res, Dept Genet & Plant Breeding, D-50829 Cologne, Germany
[2] Wageningen Univ, Genet Lab, NL-6708 PB Wageningen, Netherlands
来源：
PLANT CELL | 2010年 / 22卷 / 06期
关键词：
MESSENGER-RNA; PROTEIN; GENE; THALIANA; DOMAIN; EXPRESSION; INTERACT; COMPLEX; PLANTS; TRANSFORMATION;
D O I：
10.1105/tpc.110.074674
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
ABSCISIC ACID INSENSITIVE3 (ABI3) is a major regulator of seed maturation in Arabidopsis thaliana. We detected two ABI3 transcripts, ABI3-alpha and ABI3-beta, which encode full-length and truncated proteins, respectively. Alternative splicing of ABI3 is developmentally regulated, and the ABI3-beta transcript accumulates at the end of seed maturation. The two ABI3 transcripts differ by the presence of a cryptic intron in ABI3-alpha, which is spliced out in ABI3-beta. The suppressor of abi3-5 (sua) mutant consistently restores wild-type seed features in the frameshift mutant abi3-5 but does not suppress other abi3 mutant alleles. SUA is a conserved splicing factor, homologous to the human protein RBM5, and reduces splicing of the cryptic ABI3 intron, leading to a decrease in ABI3-beta transcript. In the abi3-5 mutant, ABI3-beta codes for a functional ABI3 protein due to frameshift restoration.
引用
收藏
页码：1936 / 1946
页数：11
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