Microwave Irradiation-assisted RNA Extraction from Woody Tissues for Plant Virus Detection

被引：0

作者：

Van Duong, Thanh ^{[1
]}

Shin, Dong-Il ^{[1
]}

Park, Hee-Sung ^{[1
]}

机构：

[1] Catholic Univ Daegu, Dept Biotechnol, Kyungsan 712702, South Korea

来源：

PLANT PATHOLOGY JOURNAL | 2010年 / 26卷 / 03期

关键词：

microwave irradiation; RNA extraction; RT-PCR; plant virus; IN-SITU HYBRIDIZATION; STEM-GROOVING-VIRUS; RT-PCR; DNA;

D O I：

暂无

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Plant tissue homogenization using a mortar or mechanical equipment has been the preferred method for obtaining high yields of total RNA; this method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization, allows for the cost-effective and rapid generation of intact RNA from apple cane shavings and the reliable detection of apple virus by RT-PCR.

引用

页码：286 / 288

页数：3

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