Effect of Yiqi Huoxue Granules on Platelet Activation Induced by Thrombin

被引：2
作者：
Lei, Zhen [1 ,2 ]
Gao, Shuibo [1 ,2 ]
Wang, Xinzhou [1 ,2 ]
Gao, Haixia [1 ,2 ]
Han, Yongjun [1 ,2 ]
Wang, Zhentao [3 ]
Wu, Hong [1 ,2 ,3 ]
机构：
[1] Henan Univ Chinese Med, Cent Lab, Henan Prov Hosp Tradit Chinese Med, Affiliated Hosp 2, Zhengzhou 450002, Peoples R China
[2] Henan Univ Chinese Med, Lab Cell Imaging, Zhengzhou 450002, Peoples R China
[3] Henan Univ Chinese Med, Inst Cardiovasc Dis, Zhengzhou 450002, Peoples R China
来源：
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2021年 / 2021卷
基金：
中国国家自然科学基金;
关键词：
ARTERIAL THROMBOSIS; ANTIPLATELET; PHOSPHORYLATION; INHIBITION; RECEPTORS; THERAPY;
D O I：
10.1155/2021/6622848
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
Objective. To study the effects of Yiqi Huoxue (YQHX) granules on platelet activation and aggregation induced by thrombin. Methods. The effect of YQHX on platelet aggregation rate was detected by platelet aggregation instrument; the effect of YQHX on thrombosis time was observed by the mouse mesentery thrombosis model. DAMI cells were induced to transform into platelet-like granules using PMA, and the effects of SCH (PAR-1 inhibitor) on thrombin-induced changes in platelet intracellular calcium concentration, PAR-1 protein expression, and phosphorylation of MAPK were examined. Results. Compared with the control group, the platelet aggregation rate, PAR-1 protein expression, phosphorylation of ERK1/2, and p38 protein in the YQHX group decreased (P<0.05), and there was no significant difference between the YQHX + SCH group and YQHX group (P>0.05). Conclusion. YQHX suppresses the platelet activation induced by thrombin by inhibiting PAR-1 expression.
引用
收藏
页数：8
相关论文
共 50 条
[21]   Functional and binding studies of gallic acid showing platelet aggregation inhibitory effect as a thrombin inhibitor [J].
Zhang, Yuxin ;
Wang, Xing ;
Lu, Binan ;
Gao, Yanbin ;
Zhang, Yanling ;
Li, Yatong ;
Niu, Hongjuan ;
Fan, Lu ;
Pang, Zongran ;
Qiao, Yanjiang .
CHINESE HERBAL MEDICINES, 2022, 14 (02) :303-309
[22]   Molecular mechanisms of platelet activation and aggregation induced by breast cancer cells [J].
Zara, Marta ;
Canobbio, Ilaria ;
Visconte, Caterina ;
Canino, Jessica ;
Torti, Mauro ;
Guidetti, Gianni Francesco .
CELLULAR SIGNALLING, 2018, 48 :45-53
[23]   Underlying mechanism and specific prevention of hemolysis-induced platelet activation [J].
Gremmel, Thomas ;
Fedrizzi, Simone ;
Weigel, Guenter ;
Eichelberger, Beate ;
Panzer, Simon .
PLATELETS, 2017, 28 (06) :555-559
[24]   Glycated albumin modulates platelet susceptibility to flow induced activation and aggregation [J].
Rubenstein, David A. ;
Yin, Wei .
PLATELETS, 2009, 20 (03) :206-215
[25]   A novel selective inhibitor to thrombin-induced platelet aggregation purified from the leech Whitmania pigra [J].
Liu, Xuan ;
Wang, Caihui ;
Ding, Xue ;
Liu, Xiaodong ;
Li, Qian ;
Kong, Yi .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2016, 473 (01) :349-354
[26]   Apocynin Prevents Abnormal Megakaryopoiesis and Platelet Activation Induced by Chronic Stress [J].
Sandrini, Leonardo ;
Ieraci, Alessandro ;
Amadio, Patrizia ;
Popoli, Maurizio ;
Tremoli, Elena ;
Barbieri, Silvia S. .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2017, 2017
[27]   A Platelet Function Modulator of Thrombin Activation Is Causally Linked to Cardiovascular Disease and Affects PAR4 Receptor Signaling [J].
Rodriguez, Benjamin A. T. ;
Bhan, Arunoday ;
Beswick, Andrew ;
Elwood, Peter C. ;
Niiranen, Teemu J. ;
Salomaa, Veikko ;
Tregouet, David-Alexandre ;
Morange, Pierre-Emmanuel ;
Civelek, Mete ;
Ben-Shlomo, Yoav ;
Schlaeger, Thorsten ;
Chen, Ming-Huei ;
Johnson, Andrew D. .
AMERICAN JOURNAL OF HUMAN GENETICS, 2020, 107 (02) :211-221
[28]   Blood clot properties, thrombin generation, and platelet activation in patients with dysglycemia and established atherosclerotic cardiovascular disease: The CASCARA study [J].
Siniarski, Aleksander ;
Golebiowska-Wiatrak, Renata ;
Malinowski, Krzysztof P. ;
Gajos, Grzegorz .
POLISH HEART JOURNAL-KARDIOLOGIA POLSKA, 2024, 82 (06) :617-624
[29]   The effect of P2Y-mediated platelet activation on the release of VEGF and endostatin from platelets [J].
Bambace, Nadia M. ;
Levis, Jamie E. ;
Holmes, Chris E. .
PLATELETS, 2010, 21 (02) :85-93
[30]   Glycolysis maintains AMPK activation in sorafenib-induced Warburg effect [J].
Guo, Sijia ;
Zhang, Chenhao ;
Zeng, Haiou ;
Xia, Yantao ;
Weng, Chenghao ;
Deng, Yichen ;
Wang, Luda ;
Wang, Huan .
MOLECULAR METABOLISM, 2023, 77
12345