Functionalization of Cellulose Acetate Fibers with Engineered Cutinases

被引：21

作者：

Matama, Teresa ^{[1
]}

Araujo, Rita ^{[1
]}

Guebitz, Georg M. ^{[2
]}

Casal, Margarida ^{[3
]}

Cavaco-Paulo, Artur ^{[1
]}

机构：

[1] Univ Minho, Text Engn Dept, P-4800058 Guimaraes, Portugal

[2] Graz Univ Technol, Dept Environm Biotechnol, A-8010 Graz, Austria

[3] Univ Minho, Dept Biol, CBMA Ctr Mol & Environm Biol, P-4710057 Braga, Portugal

来源：

BIOTECHNOLOGY PROGRESS | 2010年 / 26卷 / 03期

关键词：

carbohydrate esterase; functionalization of polymers; cellulose-binding domain; biodegradable; ACETYLXYLAN ESTERASE; SURFACE MODIFICATION; INSOLUBLE CELLULOSE; TRICHODERMA-REESEI; LINKER; PURIFICATION; NMR;

D O I：

10.1002/btpr.364

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present work, we describe for the first time the specific role of cutinase on surface modification of cellulose acetate fibers. Cutinase exhibits acetyl esterase activity on diacetate and triacetate of 0.010 U and 0.007 U, respectively. An increase on the hydroxyl groups at the fiber surface of 25% for diacetate and 317% for triacetate, after a 24 h treatment, is estimated by an indirect assay. Aiming at further improvement of cutinase affinity toward cellulose acetate, chimeric cutinases are genetically engineered by fusing the 3'-end coding sequence with a bacterial or a fungal carbohydrate-binding module and varying the linker DNA sequence. A comparative analysis of these genetic constructions is presented showing that, the superficial regeneration of cellulose hydrophilicity and reactivity on highly substituted cellulose acetates is achieved by chimeric cutinases. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 26: 636-643, 2010

引用

页码：636 / 643

页数：8