Microbial colonization is required for normal neurobehavioral development in zebrafish

被引：83

作者：

Phelps, Drake ^{[1
]}

Brinkman, Nichole E. ^{[2
]}

Keely, Scott P. ^{[2
]}

Anneken, Emily M. ^{[2
]}

Catron, Tara R. ^{[1
]}

Betancourt, Doris ^{[3
]}

Wood, Charles E. ^{[4
]}

Espenschied, Scott T. ^{[5
]}

Rawls, John F. ^{[5
]}

Tal, Tamara ^{[4
]}

机构：

[1] US EPA, ORISE, ORD, NHEERL,ISTD, Res Triangle Pk, NC USA

[2] US EPA, ORD, NERL, SED, Cincinnati, OH 45268 USA

[3] US EPA, ORD, NRMRL, AEMD, Res Triangle Pk, NC USA

[4] US EPA, ORD, NHEERL, ISTD, Res Triangle Pk, NC 27709 USA

[5] Duke Univ, Dept Mol Genet & Microbiol, Durham, NC USA

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

GUT MICROBIOTA; BEHAVIOR; RESPONSES; ANXIETY; BRAIN; MACROPHAGES; MODULATION; ADULTHOOD; EXPOSURE; SEQUENCE;

D O I：

10.1038/s41598-017-10517-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Changes in resident microbiota may have wide-ranging effects on human health. We investigated whether early life microbial disruption alters neurodevelopment and behavior in larval zebrafish. Conventionally colonized, axenic, and axenic larvae colonized at 1 day post fertilization (dpf) were evaluated using a standard locomotor assay. At 10 dpf, axenic zebrafish exhibited hyperactivity compared to conventionalized and conventionally colonized controls. Impairment of host colonization using antibiotics also caused hyperactivity in conventionally colonized larvae. To determine whether there is a developmental requirement for microbial colonization, axenic embryos were serially colonized on 1, 3, 6, or 9 dpf and evaluated on 10 dpf. Normal activity levels were observed in axenic larvae colonized on 1-6 dpf, but not on 9 dpf. Colonization of axenic embryos at 1 dpf with individual bacterial species Aeromonas veronii or Vibrio cholerae was sufficient to block locomotor hyperactivity at 10 dpf. Exposure to heat-killed bacteria or microbe-associated molecular patterns pam3CSK4 or Poly(I:C) was not sufficient to block hyperactivity in axenic larvae. These data show that microbial colonization during early life is required for normal neurobehavioral development and support the concept that antibiotics and other environmental chemicals may exert neurobehavioral effects via disruption of host-associated microbial communities.

引用

页数：13

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