Antioxidant activity of extracts from Du-zhong (Eucommia ulmoides) toward various lipid peroxidation models in vitro

被引:236
作者
Yen, GC [1 ]
Hsieh, CL [1 ]
机构
[1] Natl Chung Hsing Univ, Dept Food Sci, Taichung 402, Taiwan
关键词
Du-zhong tea; water extracts; antioxidant activity; lipid peroxidation; free radical;
D O I
10.1021/jf9800458
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The antioxidant effect of extracts from leaves, raw cortex, and roasted cortex of Du-zhong was evaluated using various lipid peroxidation models. The inhibitory activity of extracts of Du-zhong (200 mu g/mL) on the peroxidation of linoleic acid measured by thiocyanate method followed the order leaves (99.9%) > roasted cortex (95.9%) > raw cortex (77.2%) at 60 h of incubation. The IC20 for leaves, roasted cortex, and raw cortex on the peroxidation of liposome, induced by Fe3+/H2O2/ascorbic acid, was <0.06, 0.24, and 0.81 mg/mL, respectively. The thiobarbituric acid reactive substances values for leaves, roasted cortex, and raw cortex were 0.12, 1.54, and 1.81 mu mol of malondialdehyde/mg of protein in enzyme-mediated microsomal peroxidation and 0.08, 0.69, and 0.88 mu mol of malondialdehyde/mg of protein in the nonenzyme-mediated microsomal peroxidation, respectively. The antioxidant activity of extracts of Du-zhong correlates to their polyphenol content. The results presented herein indicate that extracts of Du-zhong leaves may be useful in inhibiting membrane lipid peroxidation and preventing free radical-linked disease.
引用
收藏
页码:3952 / 3957
页数:6
相关论文
共 43 条
[1]   OXIDANTS, ANTIOXIDANTS, AND THE DEGENERATIVE DISEASES OF AGING [J].
AMES, BN ;
SHIGENAGA, MK ;
HAGEN, TM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :7915-7922
[2]  
[Anonymous], 1994, Antioxidants in Nutrition, Health and Disease
[3]  
Aruoma O.I., 1991, FREE RADICALS FOOD A, P173
[4]   EVALUATION OF THE ANTIOXIDANT AND PROOXIDANT ACTIONS OF GALLIC ACID AND ITS DERIVATIVES [J].
ARUOMA, OI ;
MURCIA, A ;
BUTLER, J ;
HALLIWELL, B .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (11) :1880-1885
[5]  
Buege J A, 1978, Methods Enzymol, V52, P302
[6]   OXYRADICALS AND CANCER [J].
CERUTTI, PA .
LANCET, 1994, 344 (8926) :862-863
[7]   LIPOSOMES AS MEMBRANE MODEL FOR STUDY OF LIPID-PEROXIDATION [J].
CHATTERJEE, SN ;
AGARWAL, S .
FREE RADICAL BIOLOGY AND MEDICINE, 1988, 4 (01) :51-72
[8]   MOLECULAR MEDICINE - AGING, CANCER, AND MITOCHONDRIAL DETERIORATION [J].
COTTON, DWK ;
ROGERS, S .
LANCET, 1993, 341 (8840) :281-282
[9]   REACTIVE SPECIES AND THEIR ACCUMULATION ON RADICAL-DAMAGED PROTEINS [J].
DEAN, RT ;
GIESEG, S ;
DAVIES, MJ .
TRENDS IN BIOCHEMICAL SCIENCES, 1993, 18 (11) :437-441
[10]  
DIPLOCK AT, 1994, CANCER RES, V54, pS1952