Suppression of Cucumber Green Mottle Mosaic Virus Infection by Boron Application: From the Perspective of Nutrient Elements and Carbohydrates

被引：2

作者：

Bi, Xinyue ^{[1
]}

Guo, Huiyan ^{[1
]}

Li, Xiaodong ^{[1
,2
]}

Jiang, Dong ^{[3
]}

Dong, Haonan ^{[1
]}

Zhang, Yingying ^{[1
]}

An, Mengnan ^{[1
]}

Xia, Zihao ^{[1
]}

Wang, Zhiping ^{[1
]}

Wu, Yuanhua ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Plant Protect, Liaoning Key Lab Plant Pathol y, Shenyang 110866, Peoples R China

[2] Natl Forestry & Grassland Adm, Ctr Biol Disaster Prevent & Control, Shenyang 110034, Peoples R China

[3] Liaoning Prov Green Agr Technol Ctr, Shenyang 110034, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2022年 / 70卷 / 39期

关键词：

watermelon; boron; CGMMV; nutrient element; sugar; cell wall polysaccharide; PLANTS; TOBACCO; METABOLISM; EXPRESSION; LEAVES; CHLOROPHYLL; DEFICIENCY; COMPONENTS; RESISTANCE; TRANSPORT;

D O I：

10.1021/acs.jafc.2c03069

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Cucumber green mottle mosaic virus (CGMMV) infection causes "blood flesh" symptoms in watermelon fruits, which severely reduces yield and edibleness. However, the growth of watermelon fruits is strongly associated with boron (B), a trace element for improving fruit quality. In this study, B-gradient hydroponic experiments (B concentration: 0, 2.86, and 5.72 mg-L-1 H3BO3) and foliar-spray experiments (B concentration: 30 and 300 mg.L-1 H3BO3) were performed. We found that the B-supplement could inhibit CGMMV infection and especially relieve "blood flesh" symptoms in watermelon fruits. The nutrient element, soluble sugar, and cell wall polysaccharide contents and their metabolism- and transport-related gene expressions were determined in leaves and fruits of the watermelons in B-gradient hydroponic and foliar-spray experiments. We found that the accumulation and metabolism of nutrients and carbohydrates in cells were disrupted by CGMMV infection; however, the B-supplement could restore and maintain their homeostasis. Additionally, we uncovered that NIPS;1 and SWEET4, induced by B-application with CGMMV infection, could majorly contribute to the resistance to CGMMV infection by regulating nutrient elements and carbohydrate homeostasis. These results provided a novel insight into the molecular mechanism of B-mediated CGMMV suppression and an efficient method of B-application for the improvement of watermelon quality after CGMMV infection.

引用

页码：12270 / 12286

页数：17