Elucidation of the Anatomy of a Satiety Network: Focus on Connectivity of the Parabrachial Nucleus in the Adult Rat

被引：34

作者：

Zseli, Gyorgyi ^{[1
,2
]}

Vida, Barbara ^{[1
,3
]}

Martinez, Anais ^{[4
,5
,6
]}

Lechan, Ronald M. ^{[7
,8
]}

Khan, Arshad M. ^{[4
,5
]}

Fekete, Csaba ^{[1
,7
]}

机构：

[1] Hungarian Acad Sci, Inst Expt Med, Dept Endocrine Neurobiol, 43 Szigony St, H-1083 Budapest, Hungary

[2] Semmelweis Univ, Neurosci Doctoral Sch, Neuroendocrinol Program, H-1085 Budapest, Hungary

[3] Pazmany Peter Catholic Univ, Dept Neurosci, Fac Informat Technol, H-1088 Budapest, Hungary

[4] Univ Texas El Paso, UTEP Syst Neurosci Lab, Dept Biol Sci, El Paso, TX 79902 USA

[5] Univ Texas El Paso, Border Biomed Res Ctr, El Paso, TX 79902 USA

[6] Univ Texas El Paso, Grad Program Pathobiol, Dept Biol Sci, El Paso, TX 79902 USA

[7] Tufts Med Ctr, Tupper Res Inst, Dept Med, Div Endocrinol Diabet & Metab, Boston, MA 02111 USA

[8] Tufts Univ, Sch Med, Dept Neurosci, Boston, MA 02111 USA

来源：

JOURNAL OF COMPARATIVE NEUROLOGY | 2016年 / 524卷 / 14期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

refeeding; parabrachial nucleus; anterograde tract tracing; retrograde tract tracing; c-Fos; NEURONAL ACTIVATION; TRACTUS-SOLITARIUS; MYENTERIC NEURONS; IMMUNOREACTIVITY; EXPRESSION; HINDBRAIN; CIRCUIT; HU; IDENTIFICATION; FOREBRAIN;

D O I：

10.1002/cne.23992

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We hypothesized that brain regions showing neuronal activation after refeeding comprise major nodes in a satiety network, and tested this hypothesis with two sets of experiments. Detailed c-Fos mapping comparing fasted and refed rats was performed to identify candidate nodes of the satiety network. In addition to well-known feeding-related brain regions such as the arcuate, dorsomedial, and paraventricular hypothalamic nuclei, lateral hypothalamic area, parabrachial nucleus (PB), nucleus of the solitary tract and central amygdalar nucleus, other refeeding activated regions were also identified, such as the parastrial and parasubthalamic nuclei. To begin to understand the connectivity of the satiety network, the interconnectivity of PB with other refeeding-activated neuronal groups was studied following administration of anterograde or retrograde tracers into the PB. After allowing for tracer transport time, the animals were fasted and then refed before sacrifice. Refeeding-activated neurons that project to the PB were found in the agranular insular area; bed nuclei of terminal stria; anterior hypothalamic area; arcuate, paraventricular, and dorsomedial hypothalamic nuclei; lateral hypothalamic area; parasubthalamic nucleus; central amygdalar nucleus; area postrema; and nucleus of the solitary tract. Axons originating from the PB were observed to closely associate with refeeding-activated neurons in the agranular insular area; bed nuclei of terminal stria; anterior hypothalamus; paraventricular, arcuate, and dorsomedial hypothalamic nuclei; lateral hypothalamic area; central amygdalar nucleus; parasubthalamic nucleus; ventral posterior thalamic nucleus; area postrema; and nucleus of the solitary tract. These data indicate that the PB has bidirectional connections with most refeeding-activated neuronal groups, suggesting that short-loop feedback circuits exist in this satiety network. (C) 2016 Wiley Periodicals, Inc.

引用

页码：2803 / 2827

页数：25