Biological control of Phytophthora parasitica var. Nicotianae on tobacco seedlings with non-pathogenic binucleate Rhizoctonia fungi

Nonpathogenic binucleate Rhizoctonia fungi (BNR) controlled black shank caused by Phytophthora parasitica var, nicotianae on greenhouse-grown tobacco seedlings in styrofoam float trays. Three BNR isolates were incorporated into a soil-less mix on colonized, pulverized, sifted rice particles; colonized whole rice grains; or on pelleted tobacco seeds coated with 0.5% methyl cellulose. Five-wk-old seedlings were inoculated with zoospores of P. parasitica var. nicotianae and disease rated over 10 d. The level of protection varied with method of BNR application, ranging from 40 to 70%. Overall, control was better when BNR isolates were applied on rice inocula rather than on BNR-colonized tobacco seeds. From 15% to 80% of individual roots from seedlings grown in soil-less mix amended with BNR-colonized rice grains were colonized while only 0-20% of roots from seedlings grown from BNR-colonized tobacco seeds were colonized. Likewise, 37-100% of soil-less mix amended with BNR-colonized rice grains contained BNR's while less than 3% of soil-less mix was colonized when seedlings emerged from BNR-colonized tobacco seeds. This is the first demonstration of biocontrol of Phytophthora by BNR fungi. (C) 1998 Elsevier Science Ltd. All rights reserved.