Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins

被引：43

作者：

Ramajo, V ^{[1
]}

Oleaga, A

Casanueva, P

Hillyer, GV

Muro, A

机构：

[1] CSIC, Inst Recursos Nat & Agrobiol, Dept Patol Anim, Salamanca, Spain

[2] Univ Salamanca, Fac Farm, Parasitol Lab, E-37008 Salamanca, Spain

[3] Univ Puerto Rico, Sch Med, Lab Parasite Immunol & Pathol, Puerto Rico, Spain

来源：

VETERINARY PARASITOLOGY | 2001年 / 97卷 / 01期

关键词：

Fasciola hepatica; sheep-trematoda; FABP; vaccine;

D O I：

10.1016/S0304-4017(01)00388-0

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The current study was designed to test the immunoprophylactic properties of native (nFh12) and recombinant (rFh15) antigens from Fasciola hepatica in sheep subsequently infected with the fluke. Thirty lambs were divided into six groups according to various patterns of immunisation and times of infection and necropsy. The antigens were emulsified in Freund's adjuvant. Levels of specific anti-nFh12 and anti-rFh15 antibodies rose rapidly by 2 weeks after the first immunisation and were always significantly higher in immunised-infected sheep than in control-infected sheep. On completion of the trial there was no difference in fluke burden between groups vaccinated with either of the antigens and non-immunised controls. However, worm size and faecal egg counts were significantly diminished in the sheep vaccinated with either of the antigens, suggesting an anti-fecundity effect. This is the first report of experimental vaccination of sheep against F: hepatica with purified native and recombinant antigens related to fatty acid binding proteins. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：35 / 46

页数：12

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