Localization of gamma-glutamyl transferase activity and protein in Zea mays organs and tissues

被引：15

作者：

Masi, Antonio

Destro, Tiziana

Turetta, Loris

Varotto, Serena

Caporale, Giovanni

Ferretti, Massimo

机构：

[1] Univ Padua, Dept Biotechnol, I-35020 Legnaro, Italy

[2] Univ Padua, Dept Agron & Crop Product, I-35020 Legnaro, Italy

[3] Univ Padua, Dept Expt Vet Sci, I-35020 Legnaro, Italy

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2007年 / 164卷 / 11期

关键词：

environmental sensing; glutathione metabolism; histochemical detection; immunolocalization; oxidative stress;

D O I：

10.1016/j.jplph.2006.07.016

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Gamma-glutamyl transferase/transpeptidase (GGT, (5-L-glutamyl)-peptide: aminoacid 5-glutamyltransferase; EC 2.3.2.2.) is an ectoenzyme promoting the cleavage of the gamma-glutamyl moiety of glutathione (GSH) and gamma-glutamyl related compounds. In this work, we describe the localization of GGT by enzymehistochemical. and immunohistochemical. analysis in maize plants. Our results show that the tissue spatial distribution of GGT activity closely correlates with the localization of the GGT protein. We also demonstrate that GGT activity and protein are unevenly distributed in tissues, being higher in the epidermis and stomata, parenchyma of conductive elements and root meristem. These results can contribute to our understanding of GGT function and regulation as well as its role in glutathione metabolism. To date, these are largely unknown in plants. (C) 2006 Elsevier GmbH. All rights reserved.

引用

页码：1527 / 1535

页数：9

共 21 条

[1] Evidence for a direct link between glutathione biosynthesis and stress fefense gene expression in Arabidopsis [J].