A Subset of Equine Gastric Squamous Cell Carcinomas Is Associated With Equus Caballus Papillomavirus-2 Infection

被引：23
作者：
Alloway, Elizabeth [1 ]
Linder, Keith [1 ]
May, Susan [1 ]
Rose, Trevor [1 ]
DeLay, Josepha [2 ]
Bender, Susan [3 ]
Tucker, Alison [4 ]
Luff, Jennifer [1 ]
机构：
[1] North Carolina State Univ, Coll Vet Med, Dept Populat Hlth & Pathobiol, Raleigh, NC 27607 USA
[2] Univ Guelph, Anim Hlth Lab, Guelph, ON, Canada
[3] Univ Penn, New Bolton Ctr, Sch Vet Med, Dept Pathobiol, Kennett Sq, PA 19348 USA
[4] Rollins Anim Dis Diagnost Lab, Raleigh, NC USA
来源：
VETERINARY PATHOLOGY | 2020年 / 57卷 / 03期
关键词：
horses; squamous cell carcinoma; Equus caballus papillomavirus; oncogenic viruses; stomach; in situ hybridization; IN-SITU; ECPV-2; TYPE-2; DNA;
D O I：
10.1177/0300985820908797
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Squamous cell carcinoma (SCC) is the most common neoplasm of the equine stomach. However, the mechanisms underlying malignant transformation are unknown. As Equus caballus papillomavirus-2 (EcPV-2) is a likely cause of some genital SCCs, we hypothesized that EcPV-2 is associated with a subset of equine gastric SCCs. To this aim, we performed polymerase chain reaction (PCR) and in situ hybridization (ISH) for EcPV-2 E6/ E7 oncogenes on 11 gastric SCCs and on gastric samples from 15 control horses with no SCC. PCR for EcPV-2 was positive in 7/11 (64%) gastric SCCs; non-SCC gastric samples were all negative. Intense hybridization signals for EcPV-2 E6/E7 nucleic acid were detected by ISH within tumor cells in 5/11 (45%) gastric SCCs, including distant metastases. No hybridization signals were detected within any of the non-SCC gastric cases. This study provides support for a potential association between EcPV-2 infection and a subset of equine gastric SCC.
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收藏
页码：427 / 431
页数：5
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