Kinetic Methods for Studying DNA Glycosylases Functioning in Base Excision Repair

被引：15

作者：

Coey, Christopher T. ^{[1
]}

Drohat, Alexander C. ^{[1
,2
]}

机构：

[1] Univ Maryland, Sch Med, Baltimore, MD 21201 USA

[2] Univ Maryland, Sch Med, Marlene & Stewart Greenebaum Canc Ctr, Baltimore, MD 21201 USA

来源：

DNA REPAIR ENZYMES: STRUCTURE, BIOPHYSICS, AND MECHANISM | 2017年 / 592卷

关键词：

INTERSTRAND CROSS-LINKS; N-GLYCOSIDIC BOND; THYMINE-DNA; EXTINCTION COEFFICIENTS; ABASIC SITES; DUPLEX DNA; G.T MISPAIRS; 5-CARBOXYLCYTOSINE; ENDONUCLEASE-1; BINDING;

D O I：

10.1016/bs.mie.2017.03.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Base excision repair (BER) is a conserved and ubiquitous pathway that is initiated by DNA glycosylases, which recognize and remove damaged or mismatched nucleobases, setting the stage for restoration of the correct DNA sequence by follow-on BER enzymes. DNA glycosylases employ a nucleotide-flipping step prior to cleavage of the N-glycosyl bond, and most exhibit slow release of the abasic DNA product and/or strong product inhibition. As such, studying the catalytic mechanism of these enzymes requires care in the design, execution, and interpretation of single-and multiple-turnover kinetics experiments, which is the topic of this chapter.

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收藏

页码：357 / 376

页数：20

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