Soybean miR172c Targets the Repressive AP2 Transcription Factor NNC1 to Activate ENOD40 Expression and Regulate Nodule Initiation

被引：163

作者：

Wang, Youning ^{[1
]}

Wang, Lixiang ^{[1
,2
]}

Zou, Yanmin ^{[1
]}

Chen, Liang ^{[1
]}

Cai, Zhaoming ^{[1
,2
]}

Zhang, Senlei ^{[1
,2
]}

Zhao, Fang ^{[1
]}

Tian, Yinping ^{[1
]}

Jiang, Qiong ^{[1
,2
]}

Ferguson, Brett J. ^{[3
]}

Gresshoff, Peter M. ^{[3
]}

Li, Xia ^{[1
]}

机构：

[1] Chinese Acad Sci, Ctr Agr Resources Res, Inst Genet & Dev Biol, Key State Lab Plant Cell & Chromosome Engn, Shijiazhuang 050021, Hebei, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Univ Queensland, Ctr Integrat Legume Res, St Lucia, Qld 4072, Australia

来源：

PLANT CELL | 2014年 / 26卷 / 12期

基金：

中国国家自然科学基金;

关键词：

GENE-EXPRESSION; AGROBACTERIUM-RHIZOGENES; MEDIATED TRANSFORMATION; RESPONSE REGULATORS; SUCROSE SYNTHASE; RECEPTOR KINASE; NODULATION; MICRORNAS; SYMBIOSIS; SIGNAL;

D O I：

10.1105/tpc.114.131607

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs are noncoding RNAs that act as master regulators to modulate various biological processes by posttranscriptionally repressing their target genes. Repression of their target mRNA(s) can modulate signaling cascades and subsequent cellular events. Recently, a role for miR172 in soybean (Glycine max) nodulation has been described; however, the molecular mechanism through which miR172 acts to regulate nodulation has yet to be explored. Here, we demonstrate that soybean miR172c modulates both rhizobium infection and nodule organogenesis. miR172c was induced in soybean roots inoculated with either compatible Bradyrhizobium japonicum or lipooligosaccharide Nod factor and was highly upregulated during nodule development. Reduced activity and overexpression of miR172c caused dramatic changes in nodule initiation and nodule number. We show that soybean miR172c regulates nodule formation by repressing its target gene, Nodule Number Control1, which encodes a protein that directly targets the promoter of the early nodulin gene, ENOD40. Interestingly, transcriptional levels of miR172c were regulated by both Nod Factor Receptor1 alpha/5 alpha-mediated activation and by autoregulation of nodulation-mediated inhibition. Thus, we established a direct link between miR172c and the Nod factor signaling pathway in addition to adding a new layer to the precise nodulation regulation mechanism of soybean.

引用

页码：4782 / 4801

页数：20