Immunization of mice with recombinant S-adenosyl-l-homocysteine hydrolase protein confers protection against Brucella melitensis infection

被引：26
作者：
Yang, Yanling [1 ,2 ]
Yin, Jigang [3 ]
Guo, Dejun [4 ]
Lang, Xulong [1 ]
Wang, Xinglong [1 ,3 ]
机构：
[1] PLA, Inst 11, Acad Mil Med Sci, Changchun, Peoples R China
[2] Jilin Univ, Coll Anim Sci & Vet Med, Changchun, Jilin, Peoples R China
[3] Jilin Univ, Key Lab Zoonosis Res, Minist Educ, Inst Zoonosis, Changchun, Peoples R China
[4] Heilongjiang Bayi Agr Univ, Heilongjiang, Peoples R China
来源：
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2011年 / 61卷 / 02期
关键词：
Brucellosis; immunoproteomics; subunit vaccine; immunogenic proteins; BALB/C MICE; ABORTUS INFECTION; SUPEROXIDE-DISMUTASE; RIBOSOMAL-PROTEIN; GAMMA-INTERFERON; MARINE MAMMALS; B-MELITENSIS; IN-VITRO; VACCINE; DNA;
D O I：
10.1111/j.1574-695X.2010.00758.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Due to drawbacks of live attenuated vaccines, much attention has been focused on screening Brucella-protective antigens as subunit vaccine candidates. Here, an immunoproteomic assay was used to identify the immunogenic soluble proteins of Brucella melitensis 16M. In the present study, 27 unique immunogenic proteins were identified from the two-dimensional electrophoresis immunoblot profiles by liquid chromatography tandem MS (LC-MS/MS). From this set, the gene encoding one immunodominant protein of interest, S-adenosyl-l-homocysteine hydrolase (AdoHcyase), was expressed in Escherichia coli. The recombinant AdoHcyase induced a strong antibody response in BALB/c mice, and the polyclonal antibody could recognize a band of approximately 52 kDa in the immunoblots of soluble protein extracts from five Brucella strains. rAdoHcyase significantly stimulated the production of interferon-gamma and interleukin-2, and induced a high level of protection against B. melitensis 16M challenge at 4 weeks postchallenge. Our results indicated that rAdoHcyase could be a useful candidate for the development of subunit vaccines against B. melitensis.
引用
收藏
页码：159 / 167
页数：9
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