Oral squamous cell carcinoma detection by salivary biomarkers in a Serbian population

被引：106

作者：

Brinkmann, Ole ^{[1
]}

Kastratovic, Dragana A. ^{[2
]}

Dimitrijevic, Milovan V. ^{[2
]}

Konstantinovic, Vitomir S. ^{[3
]}

Jelovac, Drago B. ^{[3
]}

Antic, Jadranka ^{[2
]}

Nesic, Vladimir S. ^{[2
]}

Markovic, Srdjan Z. ^{[4
]}

Martinovic, Zeljko R. ^{[3
]}

Akin, David ^{[1
]}

Spielmann, Nadine ^{[1
]}

Zhou, Hui ^{[1
]}

Wong, David T. ^{[1
,5
,6
,7
]}

机构：

[1] Univ Calif Los Angeles, Sch Dent, Dent Res Inst, Los Angeles, CA 90095 USA

[2] Clin Ctr Serbia, Belgrade 1100, Serbia

[3] Univ Belgrade, Stomatol Fac, Belgrade 11000, Serbia

[4] Univ Belgrade, Fac Med, Belgrade 11000, Serbia

[5] Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Los Angeles, CA 90095 USA

[6] Univ Calif Los Angeles, David Geffen Sch Med, Div Head & Neck Surg Otolaryngol, Los Angeles, CA 90095 USA

[7] Univ Calif Los Angeles, Henry Samueli Sch Engn & Appl Sci, Los Angeles, CA 90095 USA

来源：

ORAL ONCOLOGY | 2011年 / 47卷 / 01期

关键词：

Oral cancer; Biomarkers; Salivary diagnostics; Proteome; Transcriptome; CANCER DETECTION; DISEASE; DISCOVERY; VIRUS;

D O I：

10.1016/j.oraloncology.2010.10.009

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Early detection of oral squamous cell cancer (OSCC) is the key to improve the low 5-year survival rate. Using proteomic and genomic technologies we have previously discovered and validated salivary OSCC markers in American patients. The question arises whether these biomarkers are discriminatory in cohorts of different ethnic background. Six transcriptome (DUSP1, IL8, IL1B, OAZ1, SAT1, and S100P) and three proteome (IL1B, IL8, and M2BP) biomarkers were tested on 18 early and 17 late stage OSCC patients and 51 healthy controls with quantitative PCR and ELISA. Four transcriptome (IL8, IL1B, SAT1, and S100P) and all proteome biomarkers were significantly elevated (p < 0.05) in OSCC patients. The combination of markers yielded an AUC of 0.86, 0.85 and 0.88 for OSCC total, T1-T2, and T3-T4, respectively. The sensitivity/specificity for OSCC total was 0.89/0.78, for T1-T2 0.67/0.96, and for T3-T4 0.82/0.84. In conclusion, seven of the nine salivary biomarkers (three proteins and four mRNAs) were validated and performed strongest in late stage cancer. Patient-based salivary diagnostics is a highly promising approach for OSCC detection. This study shows that previously discovered and validated salivary OSCC biomarkers are discriminatory and reproducible in a different ethnic cohort. These findings support the feasibility to implement multi-center, multi-ethnicity clinical trials towards the pivotal validation of salivary biomarkers for OSCC detection. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：51 / 55

页数：5

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