Effects of anthocyanins on β-lactoglobulin glycoxidation: a study of mechanisms and structure-activity relationship

被引：1

作者：

Wang, Ruifeng ^{[1
]}

Khalifa, Ibrahim ^{[4
]}

Du, Xia ^{[1
]}

Li, Kaikai ^{[1
]}

Xu, Yujuan ^{[3
]}

Li, Chunmei ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China

[2] Huazhong Agr Univ, Minist Educ, Environm Correlat Food Sci, Wuhan 430070, Peoples R China

[3] Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Key Lab Funct Prod, Minist Agr,Key Lab Agr Prod Proc, Guangzhou 510610, Peoples R China

[4] Benha Univ, Fac Agr, Food Technol Dept, Moshtohor 13736, Egypt

来源：

FOOD & FUNCTION | 2021年 / 12卷 / 21期

基金：

国家重点研发计划;

关键词：

END-PRODUCTS AGES; ADVANCED GLYCATION ENDPRODUCTS; WHEY-PROTEIN; CAPACITY; METHYLGLYOXAL; POLYPHENOLS; PRESSURE; CATECHIN; LYSINE; MODEL;

D O I：

10.1039/d1fo01665b

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We elucidated the underlying mechanisms of the anti-glycoxidation effects of five structurally different anthocyanins on glycated-beta-lactoglobulin (beta-Lg). The results indicated that anthocyanins structurally inhibited the formation of advanced glycation end-products, where petunidin-3-rutinoside-(p-coumaryl)-5-glucoside (Pt-Gl) exerted higher effects than those of others (p < 0.05). Through the three main steps of glycoxidation, anthocyanins trapped intermediate dicarbonyls and blocked some of the glycation sites of beta-Lg. UPLC-ESI-Q-TOF-MS characterized that these anthocyanins structurally formed mono- and di-GO/MGO adducts, and Pt-Gl formed adducts with both dicarbonyls. More importantly, Pt-Gl interacted with some of the glycation sites of beta-Lg such as Lys100, Lys101, and Arg124. Structurally, it was found that high-molecular weight anthocyanins with coumaric acid acylation seem to be better than others, which was followed by di- and mono-glycoside anthocyanins. Overall, GO/MGO-trapping and beta-Lg-anthocyanin binding are revealed as the key mechanisms of the anti-glycoxidation effects of anthocyanins on beta-Lg, which could be used as effective glycation inhibitors in protein-rich matrices.

引用

页码：10550 / 10562

页数：13

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