Effect of Gliadin on Permeability of Intestinal Biopsy Explants from Celiac Disease Patients and Patients with Non-Celiac Gluten Sensitivity

被引:139
作者
Hollon, Justin [1 ]
Puppa, Elaine Leonard [2 ]
Greenwald, Bruce [3 ]
Goldberg, Eric [3 ]
Guerrerio, Anthony [4 ]
Fasano, Alessio [5 ,6 ]
机构
[1] Naval Med Ctr Portsmouth, Dept Pediat Gastroenterol, Portsmouth, VA 23708 USA
[2] Univ Maryland, Sch Med, Baltimore, MD 21201 USA
[3] Univ Maryland, Div Gastroenterol & Hepatol, Sch Med, Baltimore, MD 21201 USA
[4] Johns Hopkins Univ, Div Pediat Gastroenterol & Nutr, Sch Med, Baltimore, MD 21205 USA
[5] Massachusetts Gen Hosp, Ctr Celiac Res, Boston, MA 02114 USA
[6] Massachusetts Gen Hosp Children, Div Pediat Gastroenterol & Nutr, Boston, MA 02114 USA
关键词
IRRITABLE-BOWEL-SYNDROME; CLINICAL-RESPONSE; BARRIER FUNCTION; TIGHT JUNCTIONS; FREE DIET; ZONULIN; INTERLEUKIN-10; PREVALENCE; MODULATOR; GUT;
D O I
10.3390/nu7031565
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Background: Intestinal exposure to gliadin leads to zonulin upregulation and consequent disassembly of intercellular tight junctions and increased intestinal permeability. We aimed to study response to gliadin exposure, in terms of barrier function and cytokine secretion, using intestinal biopsies obtained from four groups: celiac patients with active disease (ACD), celiac patients in remission (RCD), non-celiac patients with gluten sensitivity (GS) and non-celiac controls (NC). Methods: Ex-vivo human duodenal biopsies were mounted in microsnapwells and luminally incubated with either gliadin or media alone. Changes in transepithelial electrical resistance were monitored over 120 min. Media was subsequently collected and cytokines quantified. Results: Intestinal explants from all groups (ACD (n = 6), RCD (n = 6), GS (n = 6), and NC (n = 5)) demonstrated a greater increase in permeability when exposed to gliadin vs. media alone. The increase in permeability in the ACD group was greater than in the RCD and NC groups. There was a greater increase in permeability in the GS group compared to the RCD group. There was no difference in permeability between the ACD and GS groups, between the RCD and NC groups, or between the NC and GS groups. IL-10 was significantly greater in the media of the NC group compared to the RCD and GS groups. Conclusions: Increased intestinal permeability after gliadin exposure occurs in all individuals. Following gliadin exposure, both patients with gluten sensitivity and those with active celiac disease demonstrate a greater increase in intestinal permeability than celiacs in disease remission. A higher concentration of IL-10 was measured in the media exposed to control explants compared to celiac disease in remission or gluten sensitivity.
引用
收藏
页码:1565 / 1576
页数:12
相关论文
共 28 条
[1]   Gluten Causes Gastrointestinal Symptoms in Subjects Without Celiac Disease: A Double-Blind Randomized Placebo-Controlled Trial [J].
Biesiekierski, Jessica R. ;
Newnham, Evan D. ;
Irving, Peter M. ;
Barrett, Jacqueline S. ;
Haines, Melissa ;
Doecke, James D. ;
Shepherd, Susan J. ;
Muir, Jane G. ;
Gibson, Peter R. .
AMERICAN JOURNAL OF GASTROENTEROLOGY, 2011, 106 (03) :508-514
[2]   Clinical response to gluten withdrawal is not an indicator of coeliac disease [J].
Campanella, Jonia ;
Biagi, Federico ;
Bianchi, Paola Ilaria ;
Zanellati, Giovanni ;
Marchese, Alessandra ;
Corazza, Gino Roberto .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 2008, 43 (11) :1311-1314
[3]   Non-Celiac Wheat Sensitivity Diagnosed by Double-Blind Placebo-Controlled Challenge: Exploring a New Clinical Entity [J].
Carroccio, Antonio ;
Mansueto, Pasquale ;
Iacono, Giuseppe ;
Soresi, Maurizio ;
D'Alcamo, Alberto ;
Cavataio, Francesca ;
Brusca, Ignazio ;
Florena, Ada M. ;
Ambrosiano, Giuseppe ;
Seidita, Aurelio ;
Pirrone, Giuseppe ;
Rini, Giovanni Battista .
AMERICAN JOURNAL OF GASTROENTEROLOGY, 2012, 107 (12) :1898-1906
[4]   Improvement in intestinal permeability precedes morphometric recovery of the small intestine in coeliac disease [J].
Cummins, AG ;
Thompson, FM ;
Butler, RN ;
Cassidy, JC ;
Gillis, D ;
Lorenzetti, M ;
Southcott, EK ;
Wilson, PC .
CLINICAL SCIENCE, 2001, 100 (04) :379-386
[5]   Gliadin, zonulin and gut permeability: Effects on celiac and non- celiac intestinal mucosa and intestinal cell lines [J].
Drago, S ;
El Asmar, R ;
Di Pierro, M ;
Clemente, MG ;
Tripathi, A ;
Sapone, A ;
Thakar, M ;
Iacono, G ;
Carroccio, A ;
D'Agate, C ;
Not, T ;
Zampini, L ;
Catassi, C ;
Fasano, A .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 2006, 41 (04) :408-419
[6]   Host-dependent zonulin secretion causes the impairment of the small intestine barrier function after bacterial exposure [J].
El Asmar, R ;
Panigrahi, P ;
Bamford, P ;
Berti, I ;
Not, T ;
Coppa, G ;
Catassi, C ;
Fasano, A .
GASTROENTEROLOGY, 2002, 123 (05) :1607-1615
[7]   Mechanisms of disease: the role of intestinal barrier function in the pathogenesis of gastrointestinal autoimmune diseases [J].
Fasano, A ;
Shea-Donohue, T .
NATURE CLINICAL PRACTICE GASTROENTEROLOGY & HEPATOLOGY, 2005, 2 (09) :416-422
[8]   Zonulin, a newly discovered modulator of intestinal permeability, and its expression in coeliac disease [J].
Fasano, A ;
Not, T ;
Wang, WL ;
Uzzau, S ;
Berti, I ;
Tommasini, A ;
Goldblum, SE .
LANCET, 2000, 355 (9214) :1518-1519
[9]   Prevalence of celiac disease in at-risk and not-at-risk groups in the United States - A large multicenter study [J].
Fasano, A ;
Berti, I ;
Gerarduzzi, T ;
Not, T ;
Colletti, RB ;
Drago, S ;
Elitsur, Y ;
Green, PHR ;
Guandalini, S ;
Hill, ID ;
Pietzak, M ;
Ventura, A ;
Thorpe, M ;
Kryszak, D ;
Fornaroli, F ;
Wasserman, SS ;
Murray, JA ;
Horvath, K .
ARCHIVES OF INTERNAL MEDICINE, 2003, 163 (03) :286-292
[10]   Gluten sensitivity: from gut to brain [J].
Hadjivassiliou, Marios ;
Sanders, David S. ;
Gruenewald, Richard A. ;
Woodroofe, Nicola ;
Boscolo, Sabrina ;
Aeschlimann, Daniel .
LANCET NEUROLOGY, 2010, 9 (03) :318-330