Development of a label-free plasmonic gold nanoparticles aggregates sensor on the basis of charge neutralization for the detection of zearalenone

被引:23
作者
Xing, Keyu [1 ]
Peng, Juan [1 ]
Chen, Wenyao [1 ]
Fang, Bolong [1 ]
Liu, Daofeng [2 ]
Shan, Shan [3 ]
Zhang, Ganggang [4 ]
Huang, Yina [1 ]
Lai, Weihua [1 ]
机构
[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China
[2] Jiangxi Prov Ctr Dis Control & Prevent, Jiangxi Prov Key Lab Diagnosing & Tracing Foodbor, 555 East Beijing Rd, Nanchang 330029, Jiangxi, Peoples R China
[3] Jiangxi Normal Univ, Coll Life Sci, Nanchang 330012, Jiangxi, Peoples R China
[4] Jiangxi Acad Sci, Inst Microbiol, Nanchang 330096, Jiangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Dual-channel sensing platform; Zearalenone; Gold nanoparticles aggregates; Charge neutralization; SYBR Green I; SYBR-GREEN-I; BINDING; CITRATE; ASSAY;
D O I
10.1016/j.foodchem.2021.131365
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Mycotoxin contamination of corn has been considered a serious problem because it can accumulate in different organs or tissues via ingestion or skin contact and cause several health problems in humans. We have constructed a label-free, colorimetric, and fluorescence dual-channel sensing platform for the detection of zearalenone. Here, we demonstrate that plasmonic gold nanoparticles aggregates could be rapidly formed on the basis of charge neutralization by positively charged SYBR Green I. The sensing platform allowed quantitative detection as low as 0.89 mu g kg-1 and visual detection as low as 2.5 mu g kg-1. The charge neutralization strategy eliminates a major source of instability in conventional gold nanoparticles colorimetric measurements and paves the way for accurate, label-free bioanalysis.
引用
收藏
页数:7
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