Gene expression profiling of peripheral blood leukocytes identifies potential novel biomarkers of chronic obstructive pulmonary disease in current and former smokers

被引：19

作者：

Edmiston, Jeffery S. ^{[1
]}

Archer, Kellie J. ^{[2
,3
]}

Scian, Mariano J. ^{[4
]}

Joyce, Andrew R. ^{[5
]}

Zedler, Barbara K. ^{[5
]}

Murrelle, E. Lenn ^{[5
]}

机构：

[1] Altria Client Serv, Res Dev & Engn, Richmond, VA 23219 USA

[2] Virginia Commonwealth Univ, Dept Biostat, Richmond, VA USA

[3] StatSolvers LLC, Richmond, VA USA

[4] Altria Client Serv, Rem X Special Staffing, Richmond, VA USA

[5] Venebio Grp LLC, Richmond, VA USA

来源：

BIOMARKERS | 2010年 / 15卷 / 08期

关键词：

Cigarette smoking; COPD; gene expression; microarray; leukocytes; random forest; DIFFERENTIATION-ASSOCIATED PROTEIN-1; INTERLEUKIN-6; RECEPTOR; CHEMOKINE RECEPTORS; OXIDATIVE STRESS; NUCLEOTIDE-SEQUENCE; MICROARRAY ANALYSIS; AIRWAY-OBSTRUCTION; LUNG-TISSUE; COPD; INFLAMMATION;

D O I：

10.3109/1354750X.2010.512091

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Objective: To use gene expression microarrays in peripheral blood leukocytes of current and former cigarette smokers to identify differences associated with COPD. Materials and methods: Random forest modelling and a split-sample case-control approach were used to identify candidate predictors. Results: We identified 1013 genes and one smoking exposure variable that differentiated current and former smokers with or without COPD. This predictor set was reduced to a nine-gene classifier (IL6R, CCR2, PPP2CB, RASSF2, WTAP, DNTTIP2, GDAP1, LIPE and RPL14). Conclusion: These gene expression profiles represent potential biomarkers for COPD and may help increase mechanistic understanding of the disease.

引用

页码：715 / 730

页数：16

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