Upconversion nanoparticle-based fluorescence resonance energy transfer assay for organophosphorus pesticides

被引：188

作者：

Long, Qian ^{[1
]}

Li, Haitao ^{[1
]}

Zhang, Youyu ^{[1
]}

Yao, Shouzhuo ^{[1
]}

机构：

[1] Hunan Normal Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 68卷

基金：

中国国家自然科学基金; 国家教育部博士点专项基金资助;

关键词：

Upconversion nanoparticles; Gold nanoparticle; Fluorescence resonance energy transfer; Organophosphorus pesticides; ULTRASENSITIVE DETECTION; ACETYLCHOLINESTERASE ACTIVITY; LIQUID-CHROMATOGRAPHY; CARBAMATE PESTICIDES; QUANTUM DOTS; SENSOR; BIOSENSOR; SAMPLES; GAS; MICROEXTRACTION;

D O I：

10.1016/j.bios.2014.12.046

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This paper reports a novel nanosensor for organophosphorus pesticides based on the fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er upconversion nanoparticles (UCNPs) and gold nanoparticles (AuNPs). The detection mechanism is based on the facts that AuNPs quench the fluorescence of UCNPs and organophosphorus pesticides (OPs) inhibit the activity of acetylcholinesterase (AChE) which catalyzes the hydrolysis of acetylthiocholine (ATC) into thiocholine. Under the optimized conditions, the logarithm of the pesticides concentration was proportional to the inhibition efficiency. The detection limits of parathion-methyl, monocrotophos and dimethoate reached 0.67, 23, and 67 ng/L, respectively. Meanwhile, the biosensor shows good sensitivity, stability, and could be successfully applied to detection of OPs in real food samples, suggesting the biosensor has potentially extensive application clinic diagnoses assays. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：168 / 174

页数：7

共 42 条

[1] A Simple Colorimetric Enzymatic-Assay, based on immobilization of acetylcholinesterase by adsorption, for sensitive detection of organophosphorus insecticides in olive oil [J].