Integrated capillary fluorescence DNA biosensor

被引：18

作者：

Breimer, MA ^{[1
]}

Gelfand, Y ^{[1
]}

Sadik, OA ^{[1
]}

机构：

[1] SUNY Binghamton, Dept Chem, Binghamton, NY 13902 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2003年 / 18卷 / 09期

基金：

美国国家科学基金会;

关键词：

capillary fluorescence; DNA; biosensor;

D O I：

10.1016/S0956-5663(02)00248-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Covalent attachment of dsDNA molecules inside a glass capillary without the need for hybridization is described. It is shown that the glass capillary has a surface density of 2.5 X 10(13) molecules/cm(2) with specific binding capacity of 62.5%. The resulting substrate was used to develop a biosensor for determining fluorescent organic analytes and metal binding with DNA. The biosensor combines highly specific immobilization chemistry with a capillary-geometry flow cell arrangement. The results show that fluorescent dyes are retained in the dsDNA-modified surface and that exposure to concentrations of nickel and lead ions resulted in a recoverable, highly reproducible diminishment of the fluorescence intensity. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：1135 / 1147

页数：13

共 40 条

[1] Hybridization assay at a disposable electrochemical biosensor for the attomole detection of amplified human cytomegalovirus DNA [J].