共 36 条
Elucidation of cellulose accessibility, hydrolysability and reactivity as the major limitations in the enzymatic hydrolysis of cellulose
被引:56
作者:
Bansal, Prabuddha
[1
]
Vowell, Bryan J.
[1
]
Hall, Melanie
[1
,2
]
Realff, Matthew J.
[1
]
Lee, Jay H.
[1
,3
]
Bommarius, Andreas S.
[1
,4
]
机构:
[1] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
[2] Graz Univ, Dept Chem Organ & Bioorgan Chem, A-8010 Graz, Austria
[3] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea
[4] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA
关键词:
Accessibility;
Hydrolysability;
Reactivity;
Enzymatic cellulose hydrolysis;
Kinetic modeling;
KINETIC-MODEL;
CELLULASES;
BIOFUELS;
CRYSTALLINITY;
PROTEIN;
SURFACE;
D O I:
10.1016/j.biortech.2011.12.063
中图分类号:
S2 [农业工程];
学科分类号:
0828 ;
摘要:
The precipitous decline in the rates of enzymatic hydrolysis of cellulose with conversion is one of the major limitations to the commercialization of second-generation biofuel. In this work, various rate-limiting factors (fractal kinetics, changes in crystallinity, accessibility, reactivity and hydrolysable fraction, enzyme clogging, and degree of polymerization) were investigated employing experimental as well as computational studies. Model-guided experiments showed cellulose accessibility and the hydrolysable fraction of accessible substrate (a previously undefined and unreported quantity) to decrease steadily until a conversion level of nearly 70%, while cellulose reactivity, defined in terms of hydrolytic activity per amount of actively adsorbed cellulase, remained constant. Substrate depletion, accessibility and hydrolysability decrease accounted for approximately 90% of rate retardation up to 70% conversion. Faster restart rates were observed on partially converted cellulose as compared to uninterrupted hydrolysis rates, supporting an enzyme clogging phenomenon that could possibly be responsible for the additional rate decrease. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:243 / 250
页数:8
相关论文