Characterisation of multidrug-resistant Shiga toxin-producing Escherichia coli cultured from pigs in China: co-occurrence of extended-spectrum β-lactamase- and mcr-1-encoding genes on plasmids

被引:38
作者
Bai, Li [1 ]
Hurley, Daniel [2 ]
Li, Juan [3 ]
Meng, Qiong [4 ]
Wang, Juan [5 ]
Fanning, Seamus [1 ,2 ,6 ]
Xiong, Yanwen [3 ]
机构
[1] China Natl Ctr Food Safety Risk Assessment, Minist Hlth, Key Lab Food Safety Risk Assessment, Beijing, Peoples R China
[2] Univ Coll Dublin, Sch Publ Hlth Physiotherapy & Sports Sci, UCD Ctr Food Safety, Dublin 4, Ireland
[3] Chinese Ctr Dis Control & Prevent, Collaborat Innovat Ctr Diag & Treatment Infect Di, State Key Lab Infect Dis Prevent & Control, Natl Inst Communicable Dis Control & Prevent, Beijing, Peoples R China
[4] Zhejiang Prov Ctr Dis Control & Prevent, Hangzhou, Zhejiang, Peoples R China
[5] Northwest A&F Univ, Coll Vet Med, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China
[6] Univ Coll Dublin, UCD Ctr Food Safety, Room S1-04, Dublin D04 N2E5, Ireland
基金
中国国家自然科学基金;
关键词
Shiga toxin-producing Escherichia coli; STEC; Colistin resistance; Food-producing animals; mcr-1; China; MCR-1; CARBAPENEM; EMERGENCE; ANIMALS;
D O I
10.1016/j.ijantimicag.2016.06.021
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Identification of Enterobacteriaceae harbouring the plasmid-mediated transferable colistin resistance gene mcr-1 presents a new challenge to public health. The aim of this study was to characterise multidrug-resistant Shiga toxin-producing Escherichia coli (STEC) harbouring the mcr-1 gene on plasmids cultured from pigs in China. Using CHROMagar (TM) ECC plates combined with stx gene detection by PCR, 93 STEC were recovered from 326 faecal, 351 small intestine content and 326 colon content samples taken from healthy pigs in 2011 and 2012 in China. This study, in which ten colistin-resistant isolates with minimum inhibitory concentrations (MICs) of 8-12 mg/L were identified and found to be positive by PCR for the mcr-1 gene, is a follow-up to an earlier investigation. Plasmid profiling by S1-nuclease digestion followed by pulsed-field gel electrophoresis (PFGE) identified several high-molecular-weight plasmids and these were typed by PCR-based replicon typing (PBRT). Two of the ten isolates, namely STEC-CQ09 (O116:H11/CC23/ST88) and CQ10 (O2:H32/ST3628), were selected for further study as described in this report. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
引用
收藏
页码:445 / 448
页数:4
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