A dual role of linker histone H1.4 Lys 34 acetylation in transcriptional activation

被引：75

作者：

Kamieniarz, Kinga ^{[1
]}

Izzo, Annalisa ^{[1
,2
]}

Dundr, Miroslav ^{[3
]}

Tropberger, Philipp ^{[1
]}

Ozretic, Luka ^{[4
]}

Kirfel, Jutta ^{[5
]}

Scheer, Elisabeth ^{[2
]}

Tropel, Philippe ^{[2
]}

Wisniewski, Jacek R. ^{[6
]}

Tora, Laszlo ^{[2
]}

Viville, Stephane ^{[2
,7
]}

Buettner, Reinhard ^{[4
]}

Schneider, Robert ^{[1
,2
,8
]}

机构：

[1] Max Planck Inst Immunobiol & Epigenet, D-79108 Freiburg, Germany

[2] Univ Strasbourg, INSERM U 964, CNRS UMR 7104, Inst Genet & Biol Mol & Cellulaire, F-67404 Illkirch Graffenstaden, France

[3] Rosalind Franklin Univ Med & Sci, Dept Cell Biol, N Chicago, IL 60064 USA

[4] Univ Cologne, Inst Pathol, D-50924 Cologne, Germany

[5] Univ Bonn, Inst Pathol, D-53125 Bonn, Germany

[6] Max Planck Inst Biochem, Dept Prote & Signal Trasduct, D-82152 Martinsried, Germany

[7] Ctr Hosp Univ, F-67200 Strasbourg, France

[8] Univ Freiburg, BIOSS Ctr Biol Signalling Studies, D-79104 Freiburg, Germany

来源：

GENES & DEVELOPMENT | 2012年 / 26卷 / 08期

关键词：

linker histone H1; acetylation; chromatin; GCN5; transcription; NM CHROMATIN FIBER; GENOME-WIDE; HUMAN-CELLS; EXPRESSION; GENES; PHOSPHORYLATION; COMPLEXES; BROMODOMAIN; NUCLEOSOME; DEPLETION;

D O I：

10.1101/gad.182014.111

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The linker histone H1 is a key player in chromatin organization, yet our understanding of the regulation of H1 functions by post-translational modifications is very limited. We provide here the first functional characterization of H1 acetylation. We show that H1.4K34 acetylation (H1.4K34ac) is mediated by GCN5 and is preferentially enriched at promoters of active genes, where it stimulates transcription by increasing H1 mobility and recruiting a general transcription factor. H1.4K34ac is dynamic during spermatogenesis and marks undifferentiated cells such as induced pluripotent stem (iPS) cells and testicular germ cell tumors. We propose a model for H1.4K34ac as a novel regulator of chromatin function with a dual role in transcriptional activation.

引用

页码：797 / 802

页数：6

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