Biodegradable nanoparticles of partially methylated fungal poly(β-L-malic acid) as a novel protein delivery carrier

被引：20
作者：
Portilla-Arias, Jose A. [1 ]
Garcia-Alvarez, Montserrat [1 ]
Galbis, Juan A. [2 ]
Munoz-Guerra, Sebastian [1 ]
机构：
[1] Univ Politecn Cataluna, Dept Engn Quim, ETSEIB, E-08028 Barcelona, Spain
[2] Univ Seville, Dept Quim Organ & Farmaceut, Seville 41071, Spain
来源：
MACROMOLECULAR BIOSCIENCE | 2008年 / 8卷 / 06期
关键词：
biodegradable; encapsulation; hydrolysis; nanoparticles; physical absorption;
D O I：
10.1002/mabi.200700249
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The preparation of nanoparticles from 75% methylated poly( beta-L-malic acid) is described. Their degradation in aqueous environments was examined and the influence of pH and lipase on the rate of hydrolysis was evaluated. Six proteins were used to estimate the loading efficiency of the nanoparticles. The amount of protein retained in the nanoparticles was found to depend on the acid/basic character of the protein. Protein release from the loaded nanoparticles upon incubation in water under physiological conditions encompassed polymer hydrolysis an happened steadily within 3-10 d. The activity loss of entrapped alpha-chymotrypsin caused by loading and releasing depended on the method used for loading.
引用
收藏
页码：551 / 559
页数：9
相关论文
共 18 条
  • [1] Preparation and characterization of biodegradable nanoparticles based on poly(γ-glutamic acid) with L-phenylalanine as a protein carrier
    Akagi, T
    Kaneko, T
    Kida, T
    Akashi, M
    [J]. JOURNAL OF CONTROLLED RELEASE, 2005, 108 (2-3) : 226 - 236
  • [2] [Anonymous], 1997, HDB ENG POL MAT
  • [3] Nanoparticle and targeted systems for cancer therapy
    Brannon-Peppas, L
    Blanchette, JO
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 2004, 56 (11) : 1649 - 1659
  • [4] Polymers of malic acid and 3-alkylmalic acid as synthetic PHAs in the design of biocompatible hydrolyzable devices
    Cammas, S
    Béar, MM
    Moine, L
    Escalup, R
    Ponchel, G
    Kataoka, K
    Guérin, P
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1999, 25 (1-3) : 273 - 282
  • [5] Poly(ethylene glycol) as stabilizer and emulsifying agent: a novel stabilization approach preventing aggregation and inactivation of proteins upon encapsulation in bioerodible polyester microspheres
    Castellanos, IJ
    Crespo, R
    Griebenow, K
    [J]. JOURNAL OF CONTROLLED RELEASE, 2003, 88 (01) : 135 - 145
  • [6] Encapsulation-induced aggregation and loss in activity of γ-chymotrypsin and their prevention
    Castellanos, IJ
    Cruz, G
    Crespo, R
    Griebenow, K
    [J]. JOURNAL OF CONTROLLED RELEASE, 2002, 81 (03) : 307 - 319
  • [7] CONTROLLED DELIVERY SYSTEMS FOR PROTEINS BASED ON POLY(LACTIC GLYCOLIC ACID) MICROSPHERES
    COHEN, S
    YOSHIOKA, T
    LUCARELLI, M
    HWANG, LH
    LANGER, R
    [J]. PHARMACEUTICAL RESEARCH, 1991, 8 (06) : 713 - 720
  • [8] In vivo fates of degradable poly(β-malic acid) and of its precursor, malic acid
    Domurado, D
    Fournié, P
    Braud, C
    Vert, M
    Guérin, P
    Simonnet, F
    [J]. JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, 2003, 18 (01) : 23 - 32
  • [9] Inhibition of laminin-8 in vivo using a novel poly(malic acid)-based carrier reduces glioma angiogenesis
    Fujita M.
    Khazenzon N.M.
    Ljubimov A.V.
    Lee B.-S.
    Virtanen I.
    Holler E.
    Black K.L.
    Ljubimova J.Y.
    [J]. Angiogenesis, 2006, 9 (4) : 183 - 191
  • [10] Polycefin, a new prototype of a multifunctional nonconjugate based on poly(β-L-malic acid) for drug delivery
    Lee, BS
    Fujita, M
    Khazenzon, NM
    Wawrowsky, KA
    Wachsmann-Hogiu, S
    Farkas, DL
    Black, KL
    Ljubimova, JY
    Holler, E
    [J]. BIOCONJUGATE CHEMISTRY, 2006, 17 (02) : 317 - 326
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