The influence of extracellular alkali and alkaline-earth ions on electropermeation of Saccharomyces cerevisiae

被引：11
|
作者：
Muraji, M
Tatebe, W
Berg, H [2 ]
机构：
[1] Osaka City Univ, Fac Engn, Sumiyoshi Ku, Osaka 5588585, Japan
[2] Lab Bioelectrochem, Jena, Germany
[3] Saxonian Acad Sci Leipzig, Leipzig, Germany
来源：
BIOELECTROCHEMISTRY AND BIOENERGETICS | 1998年 / 46卷 / 02期
关键词：
electropermeation; electroporation; Saccharomyces cerevisiae; Na+; K+; Mg2+; Ca2+ ions;
D O I：
10.1016/S0302-4598(98)00167-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The influence of some extracellular ions on electropermeation of Saccharomyces cerevisiae is presented. Yeast suspensions containing either NaCl, KCl, MgCl2, CaCl2, or MgSO4 were treated by application of a condenser discharge pulse. A consistent time constant was maintained for each condenser pulse. The same discharge condenser was used and the concentration of dissolved salts was adjusted so that yeast suspensions of equal electrical conductivity were prepared. The yield of electropermeated cells in the KCl, MgCl2, CaCl2 and MgSO4 suspensions was compared to the yield in the NaCl solution. Under conditions of constant field strength, the yield of electropermeated cells was larger in NaCl than in either of the other salt solutions, especially of CaCl2! (C) 1998 Elsevier Science S.A. All rights reserved.
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页码：293 / 295
页数：3
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