Nematode Genome Announcement: Draft Genome of Meloidogyne chitwoodi, an Economically Important Pest of Potato in the Pacific Northwest

被引：12

作者：

Bali, Sapinder ^{[1
,2
]}

Hu, Shengwei ^{[1
]}

Vining, Kelly ^{[3
]}

Brown, Chuck ^{[4
]}

Mojtahedi, Hassan ^{[4
]}

Zhang, Lei ^{[2
]}

Gleason, Cynthia ^{[2
]}

Sathuvalli, Vidyasagar ^{[1
]}

机构：

[1] Oregon State Univ, Hermiston Agr Res & Extens Stn, Hermiston, OR 97838 USA

[2] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA

[3] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA

[4] USDA, Prosser, WA 99350 USA

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2021年 / 34卷 / 08期

关键词：

Columbia root-knot nematode; draft genome; Illumina; PacBio; pathotype; races; SEQUENCE;

D O I：

10.1094/MPMI-12-20-0337-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Meloidogyne chitwoodi is one of the most devastating pests of potato in the U.S. Pacific Northwest (PNW). Nematode-infected tubers develop external as well as internal defects, making the potato tubers unmarketable, and resulting in economic losses. Draft genome assemblies of three M. chitwoodi genotypes-race 1, race 2 and race 1 pathotype Roza-were generated using Illumina and PacBio Sequel RS II sequencing. The final assemblies consist of 30, 39, and 38 polished contigs for race 1, race 2 and race 1 pathotype Roza, respectively, with average N-50 of 2.37 Mb and average assembled genome size of approximately 47.41 Mb. On average, 10,508 genes were annotated for each genome. Benchmarking universal single-copy ortholog (BUSCO) analysis indicated that 69.80% of the BUSCOs were complete whereas 68.80, 0.93, and 12.67% were single copy, duplicated, and fragmented, respectively. These highly contiguous genomes will enrich resources to study potato-nematode interactions and enhance breeding efforts to develop nematode-resistant potato varieties for the PNW.

引用

页码：981 / 986

页数：6

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