Enzyme-linked immunosorbent assay for nonylphenol using antibody-bound microfluid filters in vertical fluidic operation

被引：12

作者：

Matsui, Katsuhiro ^{[1
]}

Kawaji, Isao ^{[1
]}

Utsumi, Yuichi ^{[2
]}

Ukita, Yoshiaki ^{[2
]}

Asano, Toshifumi ^{[2
]}

Takeo, Masahiro ^{[1
]}

Kato, Dai-ichiro ^{[1
]}

Negoro, Seiji ^{[1
]}

机构：

[1] Univ Hyogo, Grad Sch Engn, Dept Chem & Mat Sci, Himeji, Hyogo 6712280, Japan

[2] Univ Hyogo, Lab Adv Sci & Technol Ind, Akogun, Hyogo 6781205, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2007年 / 104卷 / 04期

基金：

日本学术振兴会;

关键词：

microreactor; enzyme-linked immunosorbent assay; micro total analysis system (mu TAS); microfluid filter; nonylphenol; X-ray lithography;

D O I：

10.1263/jbb.104.347

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed an enzyme-linked immunosorbent assay for an endocrine disrupter, nonylphenol, using a microreactor composed of two reaction vessels stacked vertically through a microfluid filter. The filters constructed by deep X-ray lithography possessed 2100 through-bores (phi 40 x 200 mu m) in polymethylmethacrylate sheets (phi 3 mm), which are appropriate for biochemical reactions. Through the optimization of the immunoassay, nonylphenol was quantitatively detected at the range of 0.1-10 ng/ml.

引用

页码：347 / 350

页数：4

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