The L1TD1 Protein Interactome Reveals the Importance of Post-transcriptional Regulation in Human Pluripotency

被引：28

作者：

Emani, Maheswara Reddy ^{[1
,2
]}

Narva, Elisa ^{[1
,2
]}

Stubb, Aki ^{[1
,2
]}

Chakroborty, Deepankar ^{[1
,2
]}

Viitala, Miro ^{[1
,2
]}

Rokka, Anne ^{[1
,2
]}

Rahkonen, Nelly ^{[1
,2
]}

Moulder, Robert ^{[1
,2
]}

Denessiouk, Konstantin ^{[1
,2
]}

Trokovic, Ras ^{[3
]}

Lund, Riikka ^{[1
,2
]}

Elo, Laura L. ^{[1
,2
,4
]}

Lahesmaa, Riitta ^{[1
,2
]}

机构：

[1] Univ Turku, Turku Ctr Biotechnol, Turku 20521, Finland

[2] Abo Akad Univ, Turku 20521, Finland

[3] Univ Helsinki, Mol Neurol & Biomedicum Stem Cell Ctr, Res Programs Unit, FIN-00290 Helsinki, Finland

[4] Univ Turku, Dept Math & Stat, Turku 20014, Finland

来源：

STEM CELL REPORTS | 2015年 / 4卷 / 03期

基金：

芬兰科学院;

关键词：

EMBRYONIC STEM-CELLS; MAJOR NUCLEOLAR PROTEIN; INTERACTION NETWORK; EXPRESSION; BINDING; OCT4; RNA; DIFFERENTIATION; LOCALIZATION; COMPLEXES;

D O I：

10.1016/j.stemcr.2015.01.014

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The RNA-binding protein L1TD1 is one of the most specific and abundant proteins in pluripotent stem cells and is essential for the maintenance of pluripotency in human cells. Here, we identify the protein interaction network of L1TD1 in human embryonic stem cells (hESCs) and provide insights into the interactome network constructed in human pluripotent cells. Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation. L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development. Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency.

引用

页码：519 / 528

页数：10

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