The Influence of Microgravity on Invasive Growth in Saccharomyces cerevisiae

被引：28

作者：

Van Mulders, Sebastiaan E. ^{[2
]}

Stassen, Catherine ^{[3
]}

Daenen, Luk ^{[2
]}

Devreese, Bart ^{[3
]}

Siewers, Verena ^{[4
]}

van Eijsden, Rudy G. E. ^{[5
]}

Nielsen, Jens ^{[4
]}

Delvaux, Freddy R. ^{[2
]}

Willaert, Ronnie ^{[1
]}

机构：

[1] Vrije Univ Brussel, Struct Biol Lab, B-1050 Brussels, Belgium

[2] Katholieke Univ Leuven, Ctr Malting & Brewing, B-3001 Heverlee, Belgium

[3] Univ Ghent, Lab Prot Biochem & Biomol Engn, B-9000 Ghent, Belgium

[4] Chalmers Univ Technol, S-41296 Gothenburg, Sweden

[5] VIB Microarray Facil, Leuven, Belgium

来源：

ASTROBIOLOGY | 2011年 / 11卷 / 01期

关键词：

Microgravity; Saccharomyces cerevisiae; Adhesion; Invasive growth; Budding; Proteomics; SHEAR MODELED MICROGRAVITY; GENE-EXPRESSION; ESCHERICHIA-COLI; CANDIDA-ALBICANS; IMMUNE-SYSTEM; SPACE-FLIGHT; CELL-CELL; YEAST; FLOCCULATION; BIOFILM;

D O I：

10.1089/ast.2010.0518

中图分类号：

P1 [天文学];

学科分类号：

0704 ;

摘要：

This study investigates the effects of microgravity on colony growth and the morphological transition from single cells to short invasive filaments in the model eukaryotic organism Saccharomyces cerevisiae. Two-dimensional spreading of the yeast colonies grown on semi-solid agar medium was reduced under microgravity in the Sigma 1278b laboratory strain but not in the CMBSESA1 industrial strain. This was supported by the Sigma 1278b proteome map under microgravity conditions, which revealed upregulation of proteins linked to anaerobic conditions. The Sigma 1278b strain showed a reduced invasive growth in the center of the yeast colony. Bud scar distribution was slightly affected, with a switch toward more random budding. Together, microgravity conditions disturb spatially programmed budding patterns and generate strain-dependent growth differences in yeast colonies on semi-solid medium.

引用

页码：45 / 55

页数：11

共 72 条

[1] Abramoff M.D., 2004, Biophotonics International, V11, P36
[2] Altenburg Sara D., 2008, Genomics Proteomics & Bioinformatics, V6, P42, DOI 10.1016/S1672-0229(08)60019-4
[3] Characteristics of Flo11-dependent flocculation in Saccharomyces cerevisiae
Bayly, JC
Douglas, LM
Pretorius, IS
Bauer, FF
Dranginis, AM
[J]. FEMS YEAST RESEARCH, 2005, 5 (12) : 1151 - 1156
[4] Can genetically modified Escherichia coli with neutral buoyancy induced by gas vesicles be used as an alternative method to clinorotation for microgravity studies?
Benoit, M
Klaus, D
[J]. MICROBIOLOGY-SGM, 2005, 151 : 69 - 74
[5] Microgravity, bacteria, and the influence of motility
Benoit, Michael R.
Klaus, David M.
[J]. ADVANCES IN SPACE RESEARCH, 2007, 39 (07) : 1225 - 1232
[6] How to build a biofilm: a fungal perspective
Blankenship, Jill R.
Mitchell, Aaron P.
[J]. CURRENT OPINION IN MICROBIOLOGY, 2006, 9 (06) : 588 - 594
[7] Localization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p
Bony, M
ThinesSempoux, D
Barre, P
Blondin, B
[J]. JOURNAL OF BACTERIOLOGY, 1997, 179 (15) : 4929 - 4936
[8] Microgravity and immune responsiveness: Implications for space travel
Borchers, AT
Keen, CL
Gershwin, ME
[J]. NUTRITION, 2002, 18 (10) : 889 - 898
[9] Braus GH, 2003, MOL BIOL CELL, V14, P4272, DOI 10.1091/mbc.E03-01-0042
[10] Proteome analysis of aerobically and anaerobically grown Saccharomyces cerevisiae cells
Bruckmann, Astrid
Hensbergen, Paul J.
Balog, Crina I. A.
Deelder, Andre M.
Brandt, Raymond
Snoek, I. S. Ishtar
Steensma, H. Yde
van Heusden, G. Paul H.
[J]. JOURNAL OF PROTEOMICS, 2009, 71 (06) : 662 - 669

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