Transcriptome analysis of the post-larvae of giant freshwater prawn (Macrobrachium rosenbergii) after IAG gene knockdown with microRNA interference

被引：6

作者：

Qian, Hongli ^{[1
]}

Ma, Keyi ^{[1
,4
]}

Feng, Jianbin ^{[1
]}

Guo, Ziqi ^{[1
]}

Gong, Jinhua ^{[2
]}

Chen, Huangen ^{[3
]}

Bai, Haotian ^{[1
]}

Qiu, Gaofeng ^{[1
,4
]}

机构：

[1] Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Key Lab Freshwater Aquat Genet Resources, Minist Agr & Rural Affairs, Shanghai 201306, Peoples R China

[2] Jiangsu Dinghe Aquat Technol Dev Co Ltd, Taizhou 225300, Peoples R China

[3] Jiangsu Fishery Technol Extens Ctr, Nanjing 210036, Peoples R China

[4] Shanghai Ocean Univ, Coll Fisheries & Life Sci, 999 Hucheng Huan Rd,Pudong New Area, Shanghai 201306, Peoples R China

来源：

GENERAL AND COMPARATIVE ENDOCRINOLOGY | 2022年 / 325卷

关键词：

miR-184; IAG; Macrobrachium rosenbergii; RNA interference; Transcriptome sequencing; HORMONE; IDENTIFICATION; EXPRESSION; OVARIAN;

D O I：

10.1016/j.ygcen.2022.114054

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The insulin-like androgenic gland hormone gene (IAG) of crustaceans plays pivotal roles in the regulation of sex differentiation. MicroRNAs (miRNAs) are a class of short, non-coding RNAs that function as post-transcriptional gene regulators. However, little information about the regulatory relationship between miRNA and Macro-brachium rosenbergii IAG (MrIAG) were exposed. In this study, we used the 3' untranslated region (UTR) of MrIAG to predict potential target sites of miRNAs. The results showed that miR-184 has one target site in the 3' UTR of MrIAG. Dual-luciferase report assay in vitro confirmed that miR-184 can significantly down-regulate MrIAG expression. Besides, we constructed mutant plasmids of 3' UTR of MrIAG. The result displayed that after co-transfection of mutant plasmids and miR-184 agomir, the activity of luciferase was not affected compared to the control. These results indicated that miR-184 could directly regulate MrIAG. In addition, we found that overexpression of miR-184 in M. rosenbergii can lead to significant changes in the transcription level of genes. Compared with control group, we identified 1510 differentially expressed genes (DEGs) in the miR-184 injection group. Some DEGs were involved in sex differentiation, gonad development, growth and molting were found. qRT-PCR verification was performed on eight DEGs randomly, and the results showed that the expression level of sex-, growth-, and metabolism-related genes changed significantly after MrIAG gene knockdown. Collectively, findings from this study suggest that miR-184, by mediating IAG expression, may be involved in many physio-logical processes in M. rosenbergii. The current study lays a basic understanding for short-term silencing of MrIAG with miR-184, and facilitates miRNA function analysis in M. rosenbergii in future.

引用

页数：9