Benzoylcyclopropane Derivatives from Hypoxis hemerocallidea Corms

Two monobenzoylcyclopropane (hypoxhemerol A ( 1 ) and hypoxhemeroloside G ( 2 )) and three dibenzoylcyclopropane (hypoxhemerol B ( 3 ), hypoxhemeroloside H ( 4 ), and hypoxhemeroloside I ( 5 )) derivatives were isolated from the hydro-alcoholic extract of Hypoxis hemerocallidea corms. This is the first instance where benzoylcyclopropane analogs were isolated from any natural source. Structure elucidation was mainly based on 1D- and 2D-NMR and HRESIMS data. The absolute configuration (2 R , 4 R ) of 1 was determined via NOESY NMR and experimental and calculated ECD data analyses. Compounds 1 - 5 and 11 recently reported metabolites (hypoxoside, obtuside A, interjectin, acuminoside, curcapicycloside, and hypoxhemerolosides A-F) were screened for in vitro antimicrobial activity against various bacterial and fungal strains. Curcapicycloside and acuminoside exhibited antibacterial activity against Escherichia coli with 78 and 79% inhibition at 20 mu g/mL, respectively. Hypoxhemeroloside A showed mild antifungal activity against Cryptococcus neoformans with 63% inhibition at 20 mu g/mL.