Detailed analysis of the structural changes of bone matrix during the demineralization process using Raman spectroscopy

被引：5
作者：
Timchenko, E. V. [1 ]
Zherdeva, L. A. [1 ]
Timchenko, P. E. [1 ]
Volova, L. T. [2 ]
Ponomareva, U. V. [2 ]
机构：
[1] SSAU, Moskovskoye Shosse 34, Samara 443086, Russia
[2] Samara Med Univ, Expt Med & Biotechnol Inst, Samara 443079, Russia
来源：
4TH INTERNATIONAL CONFERENCE OF PHOTONICS AND INFORMATION OPTICS, PHIO 2015 | 2015年 / 73卷
关键词：
Raman spectroscopy; cortical bone; hydroxyapatite; collagen; scanning electron microscopy;
D O I：
10.1016/j.phpro.2015.09.161
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The results of experimental research of human cortical bone tissue depending on demineralization time were represented using Raman spectroscopy. Depending on demineralization time the ratio of the mineral (PO43- and CO32-) and organic components (amide I) of bone tissue, as well as changes in the spectral regions responsible for the structural integrity of the collagen fibers in bone tissue (1200-1460 cm(-1) and 2880-3000 cm(-1)) were investigated. The observed changes show a decrease in mineral components: thus, the value of Raman band intensity at 956 and 1069 cm-1 for 5 minutes demineralization is 68.5 and 77.3 %, for 20 minutes - 55.1 and 61.1 %, for 120 minutes - 32.8 and 37 % from Raman intensity values of not demineralized tissue objects respectively. (C) 2015 The Authors. Published by Elsevier B.V.
引用
收藏
页码：221 / 227
页数：7
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