Solid-state NMR evidence for elastin-like β-turn structure in spider dragline silk

被引:92
作者
Jenkins, Janelle E. [1 ]
Creager, Melinda S. [2 ]
Butler, Emily B. [1 ]
Lewis, Randolph V. [2 ]
Yarger, Jeffery L. [1 ]
Holland, Gregory P. [1 ]
机构
[1] Arizona State Univ, Dept Chem & Biochem, Magnet Resonance Res Ctr, Tempe, AZ 85287 USA
[2] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA
关键词
ECHO DOUBLE-RESONANCE; C-13; CHEMICAL-SHIFTS; NEPHILA-CLAVIPES; SECONDARY STRUCTURE; CONFORMATIONAL CHARACTERIZATION; PROLINE; PEPTIDES; FIBERS; SUPERCONTRACTION; POLYPEPTIDES;
D O I
10.1039/c0cc00829j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Two-dimensional homo- and heteronuclear solid-state MAS NMR experiments on (13)C/(15)N-proline labeled Argiope aurantia dragline silk provide evidence for an elastin-like beta-turn structure for the repetitive Gly-Pro-Gly-X-X motif prevalent in major ampullate spidroin 2 (MaSp2).
引用
收藏
页码:6714 / 6716
页数:3
相关论文
共 33 条
  • [1] An investigation of the divergence of major ampullate silk fibers from Nephila clavipes and Argiope aurantia
    Brooks, AE
    Steinkraus, HB
    Nelson, SR
    Lewis, RV
    [J]. BIOMACROMOLECULES, 2005, 6 (06) : 3095 - 3099
  • [2] SPIDER SILK AS RUBBER
    GOSLINE, JM
    DENNY, MW
    DEMONT, ME
    [J]. NATURE, 1984, 309 (5968) : 551 - 552
  • [3] Fiber morphology of spider silk: The effects of tensile deformation
    Grubb, DT
    Jelinski, LW
    [J]. MACROMOLECULES, 1997, 30 (10) : 2860 - 2867
  • [4] Hypotheses that correlate the sequence, structure, and mechanical properties of spider silk proteins
    Hayashi, CY
    Shipley, NH
    Lewis, RV
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1999, 24 (2-3) : 271 - 275
  • [5] HINMAN MB, 1992, J BIOL CHEM, V267, P19320
  • [6] WISE NMR characterization of nanoscale heterogeneity and mobility in supercontracted Nephila clavipes spider dragline silk
    Holland, GP
    Lewis, RV
    Yarger, JL
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (18) : 5867 - 5872
  • [7] Quantifying the fraction of glycine and alanine in β-sheet and helical conformations in spider dragline silk using solid-state NMR
    Holland, Gregory P.
    Jenkins, Janelle E.
    Creager, Melinda S.
    Lewis, Randolph V.
    Yarger, Jeffery L.
    [J]. CHEMICAL COMMUNICATIONS, 2008, (43) : 5568 - 5570
  • [8] Determining secondary structure in spider dragline silk by carbon-carbon correlation solid-state NMR spectroscopy
    Holland, Gregory P.
    Creager, Melinda S.
    Jenkins, Janelle E.
    Lewis, Randolph V.
    Yarger, Jeffery L.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (30) : 9871 - 9877
  • [9] Solid-state NMR investigation of major and minor ampullate spider silk in the native and hydrated states
    Holland, Gregory P.
    Jenkins, Janelle E.
    Creager, Melinda S.
    Lewis, Randolph V.
    Yarger, Jeffery L.
    [J]. BIOMACROMOLECULES, 2008, 9 (02) : 651 - 657
  • [10] Structure of an elastin-mimetic polypeptide by solid-state NMR chemical shift analysis
    Hong, M
    Isailovic, D
    McMillan, RA
    Conticello, VP
    [J]. BIOPOLYMERS, 2003, 70 (02) : 158 - 168