Ionizing radiation and hydrogen peroxide induced oxidative DNA base damage in two L5178Y cell lines

被引：13
|
作者：
Zastawny, TH
Kruszewski, M
Olinski, R
机构：
[1] Univ Sch Med Sci, Dept Clin Biochem, PL-85092 Bydgoszcz, Poland
[2] Inst Nucl Chem & Technol, Dept Radiobiol & Hlth Protect, PL-03195 Warsaw, Poland
来源：
FREE RADICAL BIOLOGY AND MEDICINE | 1998年 / 24卷 / 7-8期
关键词：
free radical; DNA damage; oxidative base damage; radiation sensitivity; H2O2; sensitivity;
D O I：
10.1016/S0891-5849(97)00452-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Seven oxidized DNA bases were quantified, by gas GC/MS-SIM, in chromatin from gamma-rays and H2O2 treated mouse lymphoma L5178Y (LY) cells, inversely cross-sensitive to these agents. in H2O2 treated cells (2 mM, 1 h, 37 degrees C) we found more damage in LY-R cells than in LY-S cells. On the contrary, in gamma-rays (400 Gy) treated cells we found more damaged DNA bases in LY-S cells. The yield of damaged bases in control cells was similar in both cell Lines, with the exception of 80HAde and FapyGua that were found at a much higher level in LY-S cells. The yields of damaged bases were related to cellular sensitivity to damaging agent; this observation points to a relationship between DNA base damage induction, antioxidant defense system in the intracellular milieu and cell sensitivity. (C) 1998 Elsevier Science Inc.
引用
收藏
页码：1250 / 1255
页数：6
相关论文
共 50 条
  • [1] Cytotoxicity and DNA Damage Induced by Magnetic Nanoparticle Silica in L5178Y Cell
    Kang, Jin Seok
    Yum, Young Na
    Park, Sue Nie
    BIOMOLECULES & THERAPEUTICS, 2011, 19 (02) : 261 - 266
  • [2] DNA-REPAIR OF OXIDATIVE DAMAGE IN THE CELL-LINE L5178Y(S)
    RICHARDSON, SAM
    HANNIGAN, BM
    MCKENNA, PG
    INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1990, 57 (06) : 1257 - 1257
  • [3] Comparison of the effects of bleomycin and ionizing radiation in two sublines of murine lymphoma L5178Y
    Kruszewski, M
    Zaim, J
    Gradzka, W
    Szumiel, I
    NUKLEONIKA, 2001, 46 (03) : 81 - 86
  • [4] Labile iron pool correlates with iron content in the nucleus and the formation of oxidative DNA damage in mouse lymphoma L5178Y cell lines
    Kruszewski, M
    Iwanenko, T
    ACTA BIOCHIMICA POLONICA, 2003, 50 (01) : 211 - 215
  • [5] Repair of γ-ray-induced base damage in L5178Y sublines is damage type-dependent and unrelated to radiation sensitivity
    Kruszewski, M
    Zastawny, TH
    Szumiel, I
    ACTA BIOCHIMICA POLONICA, 2001, 48 (02) : 525 - 533
  • [6] Antioxidant defense system in differentially hydrogen peroxide sensitive L5178Y sublines
    Bouzyk, E
    Iwanenko, T
    Jarocewicz, N
    Kruszewski, M
    Sochanowicz, B
    Szumiel, I
    FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (04) : 697 - 704
  • [7] HYDROGEN-PEROXIDE INDUCED REPRODUCTIVE AND INTERPHASE DEATH IN 2 STRAINS OF L5178Y MURINE LYMPHOMA DIFFERING IN RADIATION SENSITIVITY
    BOUZYK, E
    BURACZEWSKA, I
    ROSIEK, O
    SOCHANOWICZ, B
    SZUMIEL, I
    RADIATION AND ENVIRONMENTAL BIOPHYSICS, 1991, 30 (02) : 105 - 116
  • [8] Induction of DNA Damage in L5178Y Cells Treated with Gold Nanoparticle
    Kang, Jin Seok
    Yum, Young Na
    Kim, Joo Hwan
    Song, Hyuna
    Jeong, Jinyoung
    Lim, Yong Taik
    Chung, Bong Hyun
    Park, Sue Nie
    BIOMOLECULES & THERAPEUTICS, 2009, 17 (01) : 92 - 97
  • [9] CELLULAR-RECOVERY IN 2 LINES OF THE L5178Y MURINE LYMPHOMA WHICH DIFFER IN THEIR SENSITIVITY TO IONIZING-RADIATION
    MCMILLAN, TJ
    EADY, JJ
    PEACOCK, JH
    STEEL, GG
    BRITISH JOURNAL OF CANCER, 1989, 59 (05) : 831 - 831
  • [10] IONIZING-RADIATION CAUSES GREATER DNA-BASE DAMAGE IN RADIATION-SENSITIVE MUTANT M10 CELLS THAN IN PARENT MOUSE LYMPHOMA L5178Y CELLS
    MORI, T
    DIZDAROGLU, M
    RADIATION RESEARCH, 1994, 140 (01) : 85 - 90
12345