Enzymatic synthesis of 2′-deoxyadenosine and 6-methylpurine-2′-deoxyriboside by Escherichia coli DH5α overexpressing nucleoside phosphorylases from Escherichia coli BL21

被引：13

作者：

Liang, Shenghua ^{[1
]}

Li, Wenzhou ^{[1
]}

Gao, Tong ^{[1
]}

Zhu, Xiangying ^{[1
]}

Yang, Guimei ^{[1
]}

Ren, Daming ^{[1
]}

机构：

[1] Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200433, Peoples R China

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2010年 / 110卷 / 02期

关键词：

2 '-Deoxyadenosine; Enzymatic synthesis; Escherichia coli; 6-Methylpurine-2 '-deoxyriboside; Nucleoside phosphorylases; SUBSTRATE-SPECIFICITY; MICROBIAL SYNTHESIS; GENE; 6-METHYLPURINE; SALMONELLA;

D O I：

10.1016/j.jbiosc.2010.01.017

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genes encoding purine nucleoside phosphorylase (deo D), uridine phosphorylase (udp) and thimidine phosphorylase (deo A) from Escherichia coli BL21 were cloned and overexpressed in E. coli DH5 alpha. The recombinant strains were employed to synthesize 2'-deoxyadenosine (dAR) and 6-methylpurine-2'-deoxyriboside (MePdR). Experimental parameters such as strains, temperature, pH, reagent concentration and cell mass were optimized. Under the optimal situation, 96% adenine was converted to dAR and 95% 6-methylpurine (MeP) was converted to MePdR in an hour, using 0.2 parts per thousand (dry wt./v) cell paste as biocatalyst. (C) 2010, The Society of Biotechnology, Japan. All rights reserved.

引用

页码：165 / 168

页数：4

共 21 条

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