Isolation and separation of HMG proteins and histones H1 and H5 and core histones by column chromatography on phosphocellulose

被引：4
|
作者：
Garg, LC
Reeck, GR
机构：
[1] Natl Inst Immunol, Gene Regulat Lab, New Delhi 110067, India
[2] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA
来源：
PROTEIN EXPRESSION AND PURIFICATION | 1998年 / 14卷 / 02期
关键词：
D O I：
10.1006/prep.1998.0927
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A single-step method to dissociate histones as well as nonhistone chromosomal proteins from chicken erythrocyte chromatin and their separation into histones H1, H5, core histones, and high mobility group proteins by column chromatography on phosphocellulose is presented. NaCl at 2.0 RI is effective in dissociating both histones and nonhistone proteins. The core histones elute as a complex. The pH is a critical factor in separating H5 from the core histones. (C) 1998 Academic Press.
引用
收藏
页码：155 / 159
页数：5
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