Antimicrobial activity of major royal jelly protein 8 and 9 of honeybee (Apis mellifera) venom

被引：6

作者：

Lee, Seonju ^{[1
]}

Lee, Kwang Sik ^{[1
]}

Ok, Min ^{[2
]}

Kim, Bo Yeon ^{[1
]}

Jin, Byung Rae ^{[1
]}

机构：

[1] Dong A Univ, Coll Nat Resources & Life Sci, Busan 49315, South Korea

[2] Nanum Bio Co Ltd, Yeongcheon 38891, South Korea

来源：

JOURNAL OF ASIA-PACIFIC ENTOMOLOGY | 2022年 / 25卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

Antimicrobial activity; Apis mellifera; Honeybee; Major royal jelly protein; Venom; BEE VENOM; LIFE-SPAN; PEPTIDE; IMMUNOTHERAPY; ANTIOXIDANT; INHIBITOR; ACTS; MECHANISMS; COMPONENTS; GLAND;

D O I：

10.1016/j.aspen.2022.101964

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Honeybee venom is a complex mixture of toxic components, including major royal jelly protein (MRJP) 8 and 9. MRJP 8 and MRJP 9 are allergens, and MRJP 8 reduces melittin-induced cell apoptosis. However, their func-tional roles are poorly understood, and their antimicrobial activities have not been determined. In this study, the antimicrobial role of MRJP 8 and MRJP 9 of honeybee (Apis mellifera) venom (AmMRJP 8 and AmMRJP 9) was demonstrated. The presence of AmMRJP 8 and AmMRJP 9 in the secreted venom was observed using antibodies against recombinant AmMRJP 8 and AmMRJP 9 produced in baculovirus-infected insect cells. Recombinant AmMRJP 8 and AmMRJP 9 exhibited an inhibitory activity against microbial serine proteases. Consistent with their inhibitory activity, they induced structural damage by binding to microbial surfaces, resulting in a broad-spectrum antimicrobial activity against bacteria and fungi. They had little effect on hemolysis. Therefore, AmMRJP 8 and AmMRJP 9 could function as antimicrobial agents in honeybee venom.

引用

页数：6

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