Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines

被引：741

作者：

Bock, Christoph ^{[1
,2
,3
,4
]}

Kiskinis, Evangelos ^{[1
,3
,5
]}

Verstappen, Griet ^{[1
,2
,3
]}

Gu, Hongcang ^{[2
]}

Boulting, Gabriella ^{[1
,3
,5
,6
]}

Smith, Zachary D. ^{[1
,2
,3
]}

Ziller, Michael ^{[1
,2
,3
]}

Croft, Gist F. ^{[7
,8
,9
,10
]}

Amoroso, Mackenzie W. ^{[7
,8
,9
,10
]}

Oakley, Derek H. ^{[7
,8
,9
,10
]}

Gnirke, Andreas ^{[2
]}

Eggan, Kevin ^{[1
,3
,5
]}

Meissner, Alexander ^{[1
,2
,3
]}

机构：

[1] Harvard Univ, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

[2] Broad Inst, Cambridge, MA 02142 USA

[3] Harvard Stem Cell Inst, Cambridge, MA 02138 USA

[4] Max Planck Inst Informat, D-66123 Saarbrucken, Germany

[5] Howard Hughes Med Inst, Chevy Chase, MD USA

[6] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[7] Columbia Univ, Jenifer Estess Lab Stem Cell Res, Dept Pathol, Ctr Motor Neuron Biol & Dis MNC,Project ALS, New York, NY 10032 USA

[8] Columbia Univ, Jenifer Estess Lab Stem Cell Res, Dept Neurol, Ctr Motor Neuron Biol & Dis MNC,Project ALS, New York, NY 10032 USA

[9] Columbia Univ, Jenifer Estess Lab Stem Cell Res, Dept Neurosci, Ctr Motor Neuron Biol & Dis MNC,Project ALS, New York, NY 10032 USA

[10] Columbia Univ, CSCI, New York, NY 10032 USA

来源：

CELL | 2011年 / 144卷 / 03期

关键词：

EMBRYONIC STEM-CELLS; METHYLATION; DIFFERENTIATION; DERIVATION; MOUSE; BIOINFORMATICS; GENERATION; BIOLOGY;

D O I：

10.1016/j.cell.2010.12.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The developmental potential of human pluripotent stem cells suggests that they can produce disease-relevant cell types for biomedical research. However, substantial variation has been reported among pluripotent cell lines, which could affect their utility and clinical safety. Such cell-line-specific differences must be better understood before one can confidently use embryonic stem (ES) or induced pluripotent stem (iPS) cells in translational research. Toward this goal we have established genome-wide reference maps of DNA methylation and gene expression for 20 previously derived human ES lines and 12 human iPS cell lines, and we have measured the in vitro differentiation propensity of these cell lines. This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines. The combination of assays yields a scorecard for quick and comprehensive characterization of pluripotent cell lines.

引用

页码：439 / 452

页数：14

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