Analysis of genetic diversity and population structure in Capsicum landraces from North Eastern India using TE-AFLP markers

被引：11
作者：
Islam, Md Aminul [1 ]
Sinha, Pratima [2 ]
Sharma, Shyam Sundar [2 ]
Negi, Madan Singh [2 ]
Neog, Bijoy [3 ]
Tripathi, Shashi Bhushan [1 ,2 ]
机构：
[1] TERI Univ, Inst Area 10, New Delhi 110070, India
[2] Energy & Resources Inst, India Habitat Ctr Complex,Lodhi Rd, New Delhi 110003, India
[3] Dibrugarh Univ, Dept Life Sci, Dibrugarh 786004, Assam, India
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 2016年 / 34卷 / 04期
关键词：
Bhut jolokia; TE-AFLP; Exotic germplasm; Population STRUCTURE; Genetic diversity; RESOURCES; PEPPER; ANNUUM; L;
D O I：
10.1007/s11105-015-0968-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
North eastern (NE) India harbours a precious germplasm repository of Capsicum in the form of various landraces. The present study was undertaken to characterise the extent of genetic variation present in different Capsicum landraces from north eastern India. A set of 171 Capsicum accessions were characterised using three-endonuclease amplified fragment length polymorphism (AFLP) markers. Out of 416 bands obtained from six primer combinations, 254 (61 %) were polymorphic. The pairwise genetic dissimilarity among accessions ranged from 0.03 to 0.97. Cluster analysis based on neighbour joining showed two major clusters. Cluster I contained most of the bhut jolokia accessions whereas cluster II contained all of the Capsicum annuum genotypes. Similar grouping was observed with population STRUCTURE analysis as well as principle coordinate analysis. Analysis of molecular variance (AMOVA) revealed 45 and 54 % variation among and within populations, respectively. This information on population structure analysis and molecular characterisation will be helpful for effective utilisation of this germplasm in Capsicum improvement programs.
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页码：869 / 875
页数：7
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