Extracellular polymeric substances diversity of biofilms grown under contrasted environmental conditions

被引：82
作者：
Ras, Monique [1 ]
Lefebvre, Dominique [1 ]
Derlon, Nicolas [2 ,3 ,4 ]
Paul, Etienne [2 ,3 ,4 ]
Girbal-Neuhauser, Elisabeth [1 ]
机构：
[1] Univ Toulouse 3, Inst Univ Technol, Lab Biol Appl Agroalimentaire & Environm, LBAE, F-32000 Auch, France
[2] Univ Toulouse, INSA, UPS, INP,LISBP, F-31077 Toulouse, France
[3] INRA, Ingn Syst Biol & Procedes UMR792, F-31400 Toulouse, France
[4] CNRS, UMR5504, F-31400 Toulouse, France
来源：
WATER RESEARCH | 2011年 / 45卷 / 04期
关键词：
Microbial biofilm; Autotrophic; Extracellular polymeric substances; Size distribution; Extraction strategy; SIZE-EXCLUSION CHROMATOGRAPHY; ACTIVATED-SLUDGE; C/N RATIO; EXTRACTION; COHESION; EPS; DETACHMENT; SYSTEMS; MATRIX;
D O I：
10.1016/j.watres.2010.11.021
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Extracellular Polymeric Substances (EPS) analysis was undertaken on three biofilms grown under different feeding conditions and offering diverging microbial activities and structural characteristics. EPS were extracted by a multi-method protocol including sonication, Tween and EDTA treatments and were characterized by size exclusion chromatography (SEC). Tween and sonication extracts presented higher EPS size diversity compared to EDTA extracts. EPS size diversity also increased with microbial functions within the biofilms and a specific 25-50 kDa cluster was identified only in extracts from biofilms presenting autotrophic activity. Another specific size cluster (180 kDa) occurred in Tween extracts provided from the mechanically stable biofilms. Such specific EPS appear as potential indicators for describing microbial and structural properties of biofilms. This study brings new elements for designing EPS fractionation and shows that size distribution analysis is an interesting tool to relate EPS diversity with macro-scale characteristics of biofilms. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1529 / 1538
页数：10
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