Electrically induced neurite outgrowth of PC12 cells on the electrode surface

被引：45

作者：

Kimura, K ^{[1
]}

Yanagida, Y ^{[1
]}

Haruyama, T ^{[1
]}

Kobatake, E ^{[1
]}

Aizawa, M ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Bioengn, Midori Ku, Yokohama, Kanagawa 226, Japan

来源：

MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING | 1998年 / 36卷 / 04期

关键词：

electrical stimulation; PC12; cell; differentiation; neurite outgrowth;

D O I：

10.1007/BF02523221

中图分类号：

TP39 [计算机的应用];

学科分类号：

081203 ; 0835 ;

摘要：

Morphological differentiation of PC12 cells cultured on an indium-tin oxide (ITO) electrode has been induced to grow neurites in the absence of nerve growth factor (NGF) by electrical stimulation. Rectangular pulse wave potentials were applied to the electrode at amplitudes of 200 mV and 400 mV with frequencies of 50 Hz, 500 Hz, and 1 kHz. The PC12 cells differentiated most prominently at 200 mV with 100 Hz. No statistically significant differences were observed among the electrically induced neurite lengths. The electrically induced differentiation was completely inhibited by a blockade of calcium influx using LaCl3. This indicates that repeated potential shift in the vicinity of a cellular membrane may stimulate morphological response, probably through calcium ion channels.

引用

收藏

页码：493 / 498

页数：6

相关论文

共 27 条

[1] INTEGRIN-MEDIATED NEURITE OUTGROWTH IN NEUROBLASTOMA-CELLS DEPENDS ON THE ACTIVATION OF POTASSIUM CHANNELS [J].

ARCANGELI, A ;

BECCHETTI, A ;

MANNINI, A ;

MUGNAI, G ;

DEFILIPPI, P ;

TARONE, G ;

DELBENE, MR ;

BARLETTA, E ;

WANKE, E ;

OLIVOTTO, M .

JOURNAL OF CELL BIOLOGY, 1993, 122 (05) :1131-1143

[2] CONSTITUTIVE UP-REGULATION OF CALCIUM-CHANNEL CURRENTS IN RAT PHEOCHROMOCYTOMA, CELLS - ROLE OF C-FOS AND C-JUN [J].

CAVALIE, A ;

BERNINGER, B ;

HAAS, CA ;

GARCIA, DE ;

LINDHOLM, D ;

LUX, HD .

JOURNAL OF PHYSIOLOGY-LONDON, 1994, 479 (01) :11-27

[3] BARIUM MODULATES C-FOS EXPRESSION AND POSTTRANSLATIONAL MODIFICATION [J].

CURRAN, T ;

MORGAN, JI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) :8521-8524

[4]

GARYANTES TK, 1992, J NEUROSCI, V12, P96

[5]

GREENBERG ME, 1985, J BIOL CHEM, V260, P4101

[6] STIMULATION OF NEURONAL ACETYLCHOLINE-RECEPTORS INDUCES RAPID GENE-TRANSCRIPTION [J].

GREENBERG, ME ;

ZIFF, EB ;

GREENE, LA .

SCIENCE, 1986, 234 (4772) :80-83

[7] ESTABLISHMENT OF A NORADRENERGIC CLONAL LINE OF RAT ADRENAL PHEOCHROMOCYTOMA CELLS WHICH RESPOND TO NERVE GROWTH-FACTOR [J].

GREENE, LA ;

TISCHLER, AS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (07) :2424-2428

[8] MAP kinase activation by flow in endothelial cells - Role of beta 1 integrins and tyrosine kinases [J].

Ishida, T ;

Peterson, TE ;

Kovach, NL ;

Berk, BC .

CIRCULATION RESEARCH, 1996, 79 (02) :310-316

[9] ELECTRICALLY PROMOTED PROTEIN-PRODUCTION BY MAMMALIAN-CELLS CULTURED ON THE ELECTRODE SURFACE [J].

KOJIMA, J ;

SHINOHARA, H ;

IKARIYAMA, Y ;

AIZAWA, M ;

NAGAIKE, K ;

MORIOKA, S .

BIOTECHNOLOGY AND BIOENGINEERING, 1992, 39 (01) :27-32

[10] ELECTRICALLY CONTROLLED PROLIFERATION OF HUMAN CARCINOMA-CELLS CULTURED ON THE SURFACE OF AN ELECTRODE [J].

KOJIMA, J ;

SHINOHARA, H ;

IKARIYAMA, Y ;

AIZAWA, M ;

NAGAIKE, K ;

MORIOKA, S .

JOURNAL OF BIOTECHNOLOGY, 1991, 18 (1-2) :129-140