Development of monoclonal antibody sandwich-ELISA for determination of beer foam-active proteins

被引：0

作者：

Kakui, T ^{[1
]}

Ishibashi, Y ^{[1
]}

Miyake, A ^{[1
]}

Terano, Y ^{[1
]}

Nakatani, K ^{[1
]}

机构：

[1] Suntory Ltd, Res Inst New Prod Dev, Shimamoto, Osaka 618, Japan

来源：

JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS | 1998年 / 56卷 / 02期

关键词：

foam-active protein; foam adhesion; malt evaluation; monoclonal;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The development of a method to determine foam-active and haze-active proteins by enzyme-linked immunosorbent assays (ELISA) based on polyclonal antibodies was previously reported. Several different monoclonal antibodies against foam-active proteins in beer were developed. These monoclonal antibodies have enabled us to develop a sandwich-ELISA for the detection of foam-active proteins. Compared with our former ELISA, this new system showed the following advantages: The detection limits for the foam-active protein determined with the new ELISA system were greatly improved, and the contents of foam-active protein quantified by new ELISA system were highly correlated with the foam adhesion on beer. This system was applied to the evaluation of foam-active proteins during the brewing process.

引用

页码：43 / 46

页数：4

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